The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200. Use in paraformaldehyde fixed cells.
Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent dilution. Titre using peptide based assay, 1:1562500
FunctionProtease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1, which decreases its transcriptional repression activity.
Tissue specificityHighly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.
Sequence similaritiesBelongs to the peptidase C48 family.
Cellular localizationNucleus. Cytoplasm. Shuttles between cytoplasm and nucleus.