Overview

  • Product nameAnti-SENP1 antibody
    See all SENP1 primary antibodies
  • Description
    Rabbit polyclonal to SENP1
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 594-643 (PQQMNGSDCG MFACKYADCI TKDRPINFTQ QHMPYFRKRM VWEILHRKLL) of human SENP1 (NP_055369).

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200. Use in paraformaldehyde fixed cells.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution. Titre using peptide based assay, 1:1562500

Target

  • FunctionProtease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1, which decreases its transcriptional repression activity.
  • Tissue specificityHighly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.
  • Sequence similaritiesBelongs to the peptidase C48 family.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • SENP 1 antibody
    • SENP1 antibody
    • SENP1_HUMAN antibody
    • Sentrin specific protease 1 antibody
    • Sentrin-specific protease 1 antibody
    • Sentrin/SUMO specific protease 1 antibody
    • Sentrin/SUMO specific protease antibody
    • Sentrin/SUMO specific protease SENP 1 antibody
    • Sentrin/SUMO specific protease SENP1 antibody
    • Sentrin/SUMO-specific protease SENP1 antibody
    • SUMO1/sentrin specific peptidase 1 antibody
    • SUMO1/sentrin specific protease 1 antibody
    • SuPr 2 antibody
    • SuPr2 antibody
    see all

Anti-SENP1 antibody images

  • Anti-SENP1 antibody (ab83321) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
    Additional bands at : 22 kDa,65 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SENP1 antibody (ab83321)

ab83321 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted May 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"