Overview

  • Product name
  • Description
    Rabbit polyclonal to SENP2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 150 and 200 of Human SENP2 (NP_067640.2)

  • Positive control
    • Whole cell lysate from HeLa or 293T cells

Properties

Applications

Our Abpromise guarantee covers the use of ab109590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 68 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway.
  • Sequence similarities
    Belongs to the peptidase C48 family.
  • Domain
    The N-terminus is necessary and sufficient for nuclear envelope targeting.
  • Post-translational
    modifications
    Polyubiquitinated; which leads to proteasomal degradation.
  • Cellular localization
    Nucleus > nuclear pore complex. Nucleus membrane. Cytoplasm. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Axam2 antibody
    • DKFZp762A2316 antibody
    • KIAA1331 antibody
    • Senp2 antibody
    • SENP2_HUMAN antibody
    • Sentrin specific protease 2 antibody
    • Sentrin-specific protease 2 antibody
    • Sentrin/SUMO specific protease SENP2 antibody
    • Sentrin/SUMO-specific protease SENP2 antibody
    • SMT3 specific isopeptidase 2 antibody
    • SMT3-specific isopeptidase 2 antibody
    • Smt3ip2 antibody
    • SUMO1/sentrin/SMT3 specific peptidase 2 antibody
    see all

Images

  • All lanes : Anti-SENP2 antibody (ab109590) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 68 kDa


    Exposure time : 3 minutes
  • Detection of SENP2 by Western Blot of Immunprecipitate.
    ab109590 at 1µg/ml staining SENP2 in HeLa whole cell lysate immunoprecipitated using ab109590 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab109590 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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