The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/250 - 1/500.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionProtease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway.
Sequence similaritiesBelongs to the peptidase C48 family.
DomainThe N-terminus is necessary and sufficient for nuclear envelope targeting.
Post-translational modificationsPolyubiquitinated; which leads to proteasomal degradation.
Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Cytoplasm. Shuttles between cytoplasm and nucleus.