The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 87 kDa.
Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division.
Immunofluorescent analysis of Control siRNA and SENP5 siRNA transfected cells (U2OS) labeling SENP5 with ab183477 at 1/1000 dilution (top panels). Cells are counter stained with DAPI (blue) and merged in lower images.