Overview

  • Product name
  • Description
    Goat polyclonal to SENP6
  • Specificity
    ab77619 is expected to recognise both reported isoforms (NP_001093879.1; NP_056386.2).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    C-KPKYEPNPHYHEN

    , corresponding to internal sequence amino acids 778-790 of Human SENP6 (NP_056386.2).

  • Positive control
    • HeLa cell nuclear lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison.
    • Tissue specificity
      Highly expressed in reproductive organs, such as testis, ovary and prostate.
    • Pathway
      Protein modification; protein sumoylation.
    • Sequence similarities
      Belongs to the peptidase C48 family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2810017C20Rik antibody
      • FLJ11355 antibody
      • FLJ11887 antibody
      • KIAA0389 antibody
      • KIAA0797 antibody
      • SENP 6 antibody
      • Senp6 antibody
      • SENP6_HUMAN antibody
      • Sentrin specific protease 6 antibody
      • Sentrin-specific protease 6 antibody
      • Sentrin/SUMO specific protease SENP6 antibody
      • Sentrin/SUMO-specific protease SENP6 antibody
      • SSP 1 antibody
      • SSP1 antibody
      • SUMO 1 specific protease 1 antibody
      • SUMO 1 specific protease antibody
      • SUMO-1-specific protease 1 antibody
      • SUMO1 specific protease 1 antibody
      • SUMO1 specific protease antibody
      • SUMO1/sentrin specific peptidase 6 antibody
      • SUSP 1 antibody
      • SUSP1 antibody
      see all

    Images

    References

    ab77619 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton 5 minutes
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 11 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Recombinant protein (E.coli produced recombinant SENP6)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    0.2 µg
    Specification
    E.coli produced recombinant SENP6
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 07 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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