Anti-SENP7 antibody (ab58422)

Overview

  • Product nameAnti-SENP7 antibody
    See all SENP7 primary antibodies
  • Description
    Rabbit polyclonal to SENP7
  • Specificityab58422 detects endogenous levels of total SENP7 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human SENP7.

  • Positive control
    • HuvEc cell extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab58422 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
ELISA 1/40000.

Target

  • FunctionProtease that deconjugates SUMO2 and SUMO3 from targeted proteins, but not SUMO1. Catalyzes the deconjugation of poly-SUMO2 and poly-SUMO3 chains. Has very low efficiency in processing full-length SUMO proteins to their mature forms.
  • Sequence similaritiesBelongs to the peptidase C48 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1707 antibody
    • Senp7 antibody
    • SENP7_HUMAN antibody
    • Sentrin specific protease antibody
    • Sentrin-specific protease 7 antibody
    • Sentrin/SUMO-specific protease SENP7 antibody
    • SSP2 antibody
    • SUMO 1 specific protease 2 antibody
    • SUMO specific protease antibody
    • SUMO-1-specific protease 2 antibody
    • SUMO1/sentrin specific peptidase 7 antibody
    • SUSP2 antibody
    see all

Anti-SENP7 antibody images

  • All lanes : Anti-SENP7 antibody (ab58422) at 1/500 dilution

    Lane 1 : HuvEc cell extracts
    Lane 2 : HuvEc cell extracts with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG (H+L)

    Predicted band size : 112 kDa
    Observed band size : 112 kDa

References for Anti-SENP7 antibody (ab58422)

ab58422 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Gel Running Conditions Reduced Denaturing (6%)
Loading amount 50 µg
Specification HEK293
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Jul 06 2016

Thank you for your enquiry. Please find the blocking protocols as below. Blocking 1. Block membrane by incubating 1 hour at room temperature or overnight at 4℃ with shaking in Blocking Solution (5% BSA, 0.05% Tween-20 in TBS (50mM Tris, 100m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"