Overview

  • Product nameAnti-SENP8 antibody
    See all SENP8 primary antibodies
  • Description
    Mouse monoclonal to SENP8
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment: NSFFHYDSHS RSNSVHAKQV AEKLEAFLGR KGDKLAFVEE KAPAQQNSYD CGMYVICNTE ALCQNFFRQQ TESLLQLLTP AYITKKRGEW KDLIATLAKK, corresponding to amino acids 113 - 212 of human SENP8 (NP_660205) with a tag

  • Positive control
    • SENP8 transfected 293T cell lysate; immunogen

Properties

Applications

Our Abpromise guarantee covers the use of ab77055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 24 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionProtease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.
  • Tissue specificityBroadly expressed, with highest levels in kidney and pancreas.
  • Sequence similaritiesBelongs to the peptidase C48 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • cysteine 2 antibody
    • DEN 1 antibody
    • DEN1 antibody
    • Deneddylase 1 antibody
    • Deneddylase-1 antibody
    • FKSG8 antibody
    • HsT17512 antibody
    • NEDD 8 specific protease 1 antibody
    • NEDD8 specific protease cysteine 2 antibody
    • NEDD8-specific protease 1 antibody
    • NEDP 1 antibody
    • NEDP1 antibody
    • Protease antibody
    • Protease, cysteine 2 antibody
    • PRSC2 antibody
    • SENP8 antibody
    • SENP8_HUMAN antibody
    • Sentrin / SUMO specific protease SENP 8 antibody
    • Sentrin-specific protease 8 antibody
    • Sentrin/SUMO-specific protease SENP8 antibody
    • SUMO sentrin specific protease family member 8 antibody
    • SUMO/sentrin peptidase family member, NEDD8 specific antibody
    • SUMO/sentrin specific peptidase family member 8 antibody
    see all

Anti-SENP8 antibody images

  • Anti-SENP8 antibody (ab77055) at 1/500 dilution + recombinant SENP8 protein fragment(the immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)- HRP conjugate at 1/5000 dilution

    Predicted band size : 24 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SENP8 antibody (ab77055) at 1/500 dilution

    Lane 1 : SENP8 transfected 293T cell lysate
    Lane 2 : non transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 24 kDa
    Observed band size : 24 kDa

References for Anti-SENP8 antibody (ab77055)

ab77055 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"