Recombinant Anti-SEP15 antibody [NCIR128A] (ab124840)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [NCIR128A] to SEP15
- Suitable for: WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SEP15 antibody [NCIR128A]
See all SEP15 primary antibodies -
Description
Rabbit monoclonal [NCIR128A] to SEP15 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse SEP15.
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Positive control
- WB: Rat liver, fetal liver, human testis, mouse testis, rat testis and LnCaP lysates; 293T transfected with N-terminal tagged human SEP15 expression vectors whole cell lysate; Human SEP15 full length recombinant protein.
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General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
NCIR128A -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
C-terminal tagged SEP15 expression vectors might be not recognized by ab124840. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa. C-terminal tagged SEP15 expression vectors might be not recognized by ab124840. |
Target
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Function
May be involved in redox reactions associated with the formation of disulfide bonds. May contribute to the quality control of protein folding in the endoplasmic reticulum. -
Tissue specificity
Higher levels in prostate and thyroid gland. -
Sequence similarities
Belongs to the selenoprotein M/SEP15 family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Endoplasmic reticulum lumen. The association with UGGT1/UGCGL1 is essential for its retention in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 9403 Human
- Entrez Gene: 93684 Mouse
- Entrez Gene: 113922 Rat
- Omim: 606254 Human
- SwissProt: O60613 Human
- SwissProt: Q9ERR7 Mouse
- SwissProt: Q923V8 Rat
- Unigene: 362728 Human
see all -
Alternative names
- 15 kDa selenoprotein antibody
- SELENOF antibody
- Selenoprotein F antibody
see all
Images
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All lanes : Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution
Lane 1 : 293T transfected with C-terminal tagged human SEP15 expression vectors, whole cell lysate at 15 µg
Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), whole cell lysate at 15 µg
Lane 3 : 293T transfected with N-terminal tagged human SEP15 expression vectors, whole cell lysate at 15 µg
Lane 4 : Human SEP15 full length recombinant protein
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST
C-terminal tagged SEP15 expression vectors might be not recognized by ab124840.
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Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution (purified) + Human testis lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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All lanes : Anti-SEP15 antibody [NCIR128A] (ab124840) at 1/1000 dilution (unpurified)
Lane 1 : Rat liver lysate
Lane 2 : Fetal liver lysate
Lane 3 : LnCaP lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 18 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab124840 has been referenced in 8 publications.
- Mideksa YG et al. A comprehensive set of ER protein disulfide isomerase family members supports the biogenesis of proinflammatory interleukin 12 family cytokines. J Biol Chem 298:102677 (2022). PubMed: 36336075
- Sonet J et al. Selenoproteome Expression Studied by Non-Radioactive Isotopic Selenium-Labeling in Human Cell Lines. Int J Mol Sci 22:N/A (2021). PubMed: 34298926
- Zheng X et al. Selenoprotein F knockout leads to glucose and lipid metabolism disorders in mice. J Biol Inorg Chem 25:1009-1022 (2020). PubMed: 32995962
- Ekoue DN et al. Correlations of SELENOF and SELENOP genotypes with serum selenium levels and prostate cancer. Prostate 78:279-288 (2018). PubMed: 29314169
- Touat-Hamici Z et al. Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines. Biochim Biophys Acta N/A:N/A (2018). Human . PubMed: 29660373
- Yim SH et al. Role of Selenof as a Gatekeeper of Secreted Disulfide-Rich Glycoproteins. Cell Rep 23:1387-1398 (2018). PubMed: 29719252
- Tsuji PA et al. The 15kDa selenoprotein and thioredoxin reductase 1 promote colon cancer by different pathways. PLoS One 10:e0124487 (2015). Human . PubMed: 25886253
- Howard MT et al. Translational redefinition of UGA codons is regulated by selenium availability. J Biol Chem 288:19401-13 (2013). WB ; Mouse . PubMed: 23696641