Anti-Separase antibody (ab52158)
Key features and details
- Rabbit polyclonal to Separase
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Separase antibody
See all Separase primary antibodies -
Description
Rabbit polyclonal to Separase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic non-phosphopeptide derived from human Separase around the phosphorylation site of serine 801 (N-S-S-P-V).
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Positive control
- human colon carcinoma, extracts from A431 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately >175 kDa (predicted molecular weight: 233 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately >175 kDa (predicted molecular weight: 233 kDa). |
Target
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Function
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. -
Sequence similarities
Belongs to the peptidase C50 family. -
Post-translational
modificationsAutocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9700 Human
- Entrez Gene: 105988 Mouse
- Omim: 604143 Human
- SwissProt: Q14674 Human
- SwissProt: P60330 Mouse
- Unigene: 153479 Human
- Unigene: 288324 Mouse
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Alternative names
- Caspase like protein ESPL1 antibody
- Caspase-like protein ESPL1 antibody
- ESP 1 antibody
see all
Images
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All lanes : Anti-Separase antibody (ab52158) at 1/500 dilution
Lane 1 : extracts from A431 cells
Lane 2 : extracts from A431 cells, with immunizing peptide
Predicted band size: 233 kDa
Observed band size: 233 kDa -
Immunohistochemical analysis of separase expression in paraffin embedded human colon carcinoma tissue using ab52158 (1:50 dilution). Left: sample untreated. Right: sample treated with immunizing peptide (negative control).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab52158 has been referenced in 1 publication.
- Bacac M et al. Securin and separase modulate membrane traffic by affecting endosomal acidification. Traffic 12:615-26 (2011). PubMed: 21272169