Overview

  • Product name
  • Description
    Rabbit polyclonal to Separase
  • Specificity
    ab52158 detects endogenous levels of total SEPARASE protein.
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non-phosphopeptide derived from human Separase around the phosphorylation site of serine 801 (N-S-S-P-V).

  • Positive control
    • human colon carcinoma, extracts from A431 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab52158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately >175 kDa (predicted molecular weight: 233 kDa).
ELISA 1/40000.

Target

  • Function
    Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
  • Sequence similarities
    Belongs to the peptidase C50 family.
  • Post-translational
    modifications
    Autocleaves. This function, which is not essential for its protease activity, is unknown.
    Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Caspase like protein ESPL1 antibody
    • Caspase-like protein ESPL1 antibody
    • ESP 1 antibody
    • ESP1 antibody
    • ESPL 1 antibody
    • Espl1 antibody
    • ESPL1_HUMAN antibody
    • Extra spindle poles like 1 antibody
    • Extra spindle poles like 1 protein antibody
    • Extra spindle poles-like 1 protein antibody
    • FLJ46492 antibody
    • KIAA0165 antibody
    • Separase antibody
    • Separin antibody
    • Similar to fission yeast cut1and gene antibody
    • SSE antibody
    see all

Images

  • All lanes : Anti-Separase antibody (ab52158) at 1/500 dilution

    Lane 1 : extracts from A431 cells
    Lane 2 : extracts from A431 cells, with immunizing peptide


    Predicted band size : 233 kDa
    Observed band size : 233 kDa
  • Immunohistochemical analysis of separase expression in paraffin embedded human colon carcinoma tissue using ab52158 (1:50 dilution). Left: sample untreated. Right: sample treated with immunizing peptide (negative control).

References

ab52158 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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