Anti-Separase (phospho S801) antibody (ab52210)

Overview

  • Product nameAnti-Separase (phospho S801) antibody
    See all Separase primary antibodies
  • Description
    Rabbit polyclonal to Separase (phospho S801)
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide designed around the phosphorylation site of serine 801 (N-S-SP-P-V) of human Separase.

  • Positive control
    • Human colon carcinoma tissue;
  • General notes


    ab52210 detects endogenous levels of Separase but only when phosphorylated at serine 801 (human) or serine 1189 (mouse).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab52210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 233 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.

Target

  • FunctionCaspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
  • Sequence similaritiesBelongs to the peptidase C50 family.
  • Post-translational
    modifications
    Autocleaves. This function, which is not essential for its protease activity, is unknown.
    Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Caspase like protein ESPL1 antibody
    • Caspase-like protein ESPL1 antibody
    • ESP 1 antibody
    • ESP1 antibody
    • ESPL 1 antibody
    • Espl1 antibody
    • ESPL1_HUMAN antibody
    • Extra spindle poles like 1 antibody
    • Extra spindle poles like 1 protein antibody
    • Extra spindle poles-like 1 protein antibody
    • FLJ46492 antibody
    • KIAA0165 antibody
    • Separase antibody
    • Separin antibody
    • Similar to fission yeast cut1and gene antibody
    • SSE antibody
    see all

Anti-Separase (phospho S801) antibody images

  • Paraffin-embedded human colon carcinoma tissue stained for Separase using ab52210 (1/100). The right hand panel represents a negative control where ab52210 was pre-incubated with the immunising (blocking) peptide.
  • All lanes : Anti-Separase (phospho S801) antibody (ab52210) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with EGF (200ng/ml, 30min). For this panel, ab52210 was pre-incubates with the immunizing (blocking) peptide.
    Lane 2 : Extracts from 293 cells treated with EGF (200ng/ml, 30min)


    Predicted band size : 233 kDa

References for Anti-Separase (phospho S801) antibody (ab52210)

ab52210 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"