Recombinant
RabMAb

Anti-SEPP1 antibody [EPR4614] (ab109514)

Overview

  • Product name
    Anti-SEPP1 antibody [EPR4614]
    See all SEPP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4614] to SEPP1
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human SEPP1.

  • Positive control
    • Human heart, HepG2 and 293T lysates; 293T cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR4614
  • Isotype
    IgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab109514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-SEPP1 antibody [EPR4614] (ab109514) at 1/1000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : HepG2 lysate
      Lane 3 : 293T lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 43 kDa
    • Flow cytometric analysis of permeabilized 293T cells, using ab109514 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

    References

    ab109514 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for contacting us.

    Unfortunately the BLAST came up "no significant homology" found between the immunogen and the chicken sequence.

    I hope this information is helpful to you. Please do not hesitate to contact us if ...

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    The immunogen sequence used to raise the Anti-SEPP1 antibody [EPR4614] (abx109514) is proprietary information. However, in order to aid your research I am able to share it with you. Please do not share or publish this information. It is strictly f...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (human colon and pancreas)
    Total protein in input
    5000 µg
    Specification
    human colon and pancreas
    Immuno-precipitation step
    Protein G
    Username

    Mr. Qing Wang

    Verified customer

    Submitted Aug 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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