The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
FunctionFilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis.
Tissue specificityExpressed at high levels in lymphoid and haematopoietic tissues.
Sequence similaritiesBelongs to the septin family.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Remains at the centrosomes and the nearby microtubules throughout mitosis. Localizes to the midbody during cytokinesis.