Overview

  • Product nameAnti-SEPT12 antibody
    See all SEPT12 primary antibodies
  • Description
    Mouse polyclonal to SEPT12
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-358 of Human FLJ25410

  • Positive control
    • Hela cells, transfected 293T cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionFilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the septin family.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. At interphase, forms a filamentous structure in the cytoplasm. During anaphase, translocates to the central spindle region and to the midbody during cytokinesis.
  • Information by UniProt
  • Database links
  • Alternative names
    • SEP12_HUMAN antibody
    • SEPT12 antibody
    • Septin-12 antibody
    • Septin12 antibody

Anti-SEPT12 antibody images

  • Immunofluorescent analysis of FLJ25410 expression in HeLa cells using 10 µg/ml ab66960.
  • All lanes : Anti-SEPT12 antibody (ab66960) at 1/1000 dilution

    Lane 1 : FLJ25410 transfected 293T cell lysate
    Lane 2 : untransfected 293T cell lysate

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

References for Anti-SEPT12 antibody (ab66960)

ab66960 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"