Overview

  • Product name
    Anti-SEPT12 antibody
  • Description
    Mouse polyclonal to SEPT12
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-358 of Human FLJ25410

  • Positive control
    • Hela cells, transfected 293T cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the septin family.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. At interphase, forms a filamentous structure in the cytoplasm. During anaphase, translocates to the central spindle region and to the midbody during cytokinesis.
  • Information by UniProt
  • Database links
  • Alternative names
    • SEP12_HUMAN antibody
    • SEPT12 antibody
    • Septin-12 antibody
    • Septin12 antibody

Images

  • Immunofluorescent analysis of FLJ25410 expression in HeLa cells using 10 µg/ml ab66960.

References

ab66960 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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