Anti-SEPT4 antibody [ARTS51] (ab10440)

Overview

  • Product nameAnti-SEPT4 antibody [ARTS51]
    See all SEPT4 primary antibodies
  • Description
    Mouse monoclonal [ARTS51] to SEPT4
  • Tested applicationsSuitable for: ELISA, WB, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human SEPT4 aa 247-266 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    NIQDNRVHCCLYFI SPFGHG

  • Positive control
    • Whole extract of transfected 293T (human embryonal kidney) cells expressing ARTS.

Properties

Applications

Our Abpromise guarantee covers the use of ab10440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 34 kDa.
ICC Use at an assay dependent dilution.

Target

  • FunctionFilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion. Isoform ARTS, but not the other isoforms, is required for the induction of cell death mediated by TGF-beta and by other apoptotic stimuli.
  • Tissue specificityWidely expressed in adult and fetal tissues with highest expression in adult brain (at protein level), heart, liver and adrenal gland and fetal heart, kidney, liver and lung. Also expressed in colorectal cancers and malignant melanomas. Expressed in platelets.
  • Sequence similaritiesBelongs to the septin family.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. In platelets, found in areas surrounding alpha-granules and Mitochondrion. Nucleus. While predominantly localized in the mitochondria under resting conditions, isoform ARTS translocates into the nucleus after TGF-beta treatment and apoptosis induction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis-related protein in the TGF-beta signaling pathway antibody
    • ARTS antibody
    • Bradeion antibody
    • Bradeion beta antibody
    • Brain protein H5 antibody
    • CE5B3 antibody
    • CE5B3 beta antibody
    • Cell division control-related protein 2 antibody
    • Cerebral protein 7 antibody
    • H5 antibody
    • hCDCREL-2 antibody
    • hucep-7 antibody
    • MART antibody
    • Peanut-like protein 2 antibody
    • PNUTL2 antibody
    • SEP 4 antibody
    • SEP4 antibody
    • Sept4 antibody
    • SEPT4_HUMAN antibody
    • Septin M antibody
    • Septin-4 antibody
    see all

References for Anti-SEPT4 antibody [ARTS51] (ab10440)

ab10440 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (mature human sperm)
Specification mature human sperm
Fixative 2% paraformaldehyde
Permeabilization Yes - 0.1% Trit-X on ice 15mins
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Apr 11 2012

DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab10440 in ICC/IF, Human sperm cells. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please su...

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Thank you for contacting us and for your interest in our products.

As far as we are aware, ab10440 has never been tested in ICC/IF. All tested applications are specified on our datasheets, and these are updated as soon as any new informati...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (colon)
Specification colon
Fixative Formaldehyde
Permeabilization Yes - Triton X
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Oct 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"