Overview

  • Product nameAnti-SEPT5 antibody [SP18]
    See all SEPT5 primary antibodies
  • Description
    Mouse monoclonal [SP18] to SEPT5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Immunoprecipitate of human brain

  • Positive control
    • Human brain extract

Properties

Applications

Our Abpromise guarantee covers the use of ab69538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). ECL

Target

  • FunctionFilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion.
  • Tissue specificityExpressed at high levels in the CNS, as well as in heart and platelets (at protein level).
  • Sequence similaritiesBelongs to the septin family.
  • Post-translational
    modifications
    In platelets, phosphorylated in response to thrombin, phorbol-12-myristate-13-acetate and collagen.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. In platelets, found in areas surrounding alpha-granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 Sep antibody
    • CDCrel 1 antibody
    • CDCREL antibody
    • CDCrel-1 antibody
    • CDCREL1 antibody
    • Cell division control related protein 1 antibody
    • Cell division control-related protein 1 antibody
    • H5 antibody
    • HCDCREL 1 antibody
    • OTTHUMP00000197271 antibody
    • OTTHUMP00000197272 antibody
    • OTTHUMP00000197273 antibody
    • OTTHUMP00000197274 antibody
    • OTTHUMP00000197275 antibody
    • OTTHUMP00000197276 antibody
    • OTTHUMP00000197396 antibody
    • OTTHUMP00000197410 antibody
    • Peanut like 1 antibody
    • Peanut like 1 homolog antibody
    • Peanut like protein 1 antibody
    • Peanut-like protein 1 antibody
    • Platelet glycoprotein Ib beta chain antibody
    • PNUTL1 antibody
    • SEPT5 antibody
    • SEPT5_HUMAN antibody
    • Septin 5 antibody
    • Septin-5 antibody
    see all

Anti-SEPT5 antibody [SP18] images

  • All lanes : Anti-SEPT5 antibody [SP18] (ab69538) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Human brain extract

    Developed using the ECL technique

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SEPT5 antibody [SP18] (ab69538)

This product has been referenced in:
  • Katagiri T  et al. Proteomic analysis of proteins expressing in regions of rat brain by a combination of SDS-PAGE with nano-liquid chromatography-quadrupole-time of flight tandem mass spectrometry. Proteome Sci 8:41 (2010). WB ; Rat . Read more (PubMed: 20659346) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Rat Tissue lysate - whole (Hypothalamus)
Specification Hypothalamus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 13 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"