The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.03 - 1 µg/ml. Predicted molecular weight: 50 kDa.
Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. May play a role in HCV RNA replication.
Belongs to the septin family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Cleavage furrow. Midbody. In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle.
ab106944 (3.8µg/ml) staining of paraffin embedded Human Tonsil following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows specific staining in dividing cells just outside a germinal centre.
Western blot - SEPT6 antibody (ab106944)
Anti-SEPT6 antibody (ab106944) at 1 µg/ml + Human testis lysate in RIPA buffer at 35 µg