Overview

  • Product name
  • Description
    Goat polyclonal to SEPT6
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:

    DEVNAFKQRKTA

    by a Cysteine residue linker, corresponding to C terminal amino acids 391-402 of Human SEPT6 (NP_665798.1; NP_055944.2; NP_665801.1).

  • Positive control
    • Human testis, thyroid gland and MOLT4 cell lysates
  • General notes


    ab106924 is expected to recognize the reported isoforms NP_665798.1, NP_055944.2 and NP_665801.1. Reported variants NP_665799.1 and NP_665798.1 represent identical protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    ab106924 is expected to recognize the reported isoforms NP_665798.1, NP_055944.2 and NP_665801.1. Reported variants NP_665799.1 and NP_665798.1 represent identical protein.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.03 - 1 µg/ml. Predicted molecular weight: 50 kDa.

Target

  • Function
    Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. May play a role in HCV RNA replication.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the septin family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Cleavage furrow. Midbody. In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0128 antibody
    • Nkrf antibody
    • RCG53214, isoform CRA_d antibody
    • RP5-876A24.2 antibody
    • SEP2 antibody
    • SEPT2 antibody
    • SEPT6 antibody
    • SEPT6/MLL FUSION GENE antibody
    • SEPT6_HUMAN antibody
    • Septin 2 antibody
    • Septin 6 antibody
    • Septin-6 antibody
    see all

Anti-SEPT6 antibody images

  • ab106944 (3.8µg/ml) staining of paraffin embedded Human Tonsil following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows specific staining in dividing cells just outside a germinal centre.

  • Anti-SEPT6 antibody (ab106944) at 1 µg/ml + Human testis lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 50 kDa

References for Anti-SEPT6 antibody (ab106944)

ab106944 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab106944.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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