Overview

  • Product nameAnti-SEPT7 antibody
    See all SEPT7 primary antibodies
  • Description
    Goat polyclonal to SEPT7
  • Specificityab79286 is expected to recognize both reported isoforms (NP_001779.3; NP_001011553.2).
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide:

    C-DNNKNKGQLTKSP

    , corresponding to internal sequence amino acids 323-335 of Human SEPT7 (NP_001779.3)

  • Positive control
    • HeLa nuclear cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab79286 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab79286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-SEPT7 antibody images

    References for Anti-SEPT7 antibody (ab79286)

    ab79286 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Peritoneal macrophages)
    Permeabilization Yes - 0,25% Triton
    Specification Peritoneal macrophages
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Fixative Formaldehyde
    Username

    Dr. Manoj B Menon

    Verified customer

    Submitted Apr 24 2015

    Abreviews
    Application Western blot
    Loading amount 10000 cells
    Gel Running Conditions Reduced Denaturing (7.5 to 16% gradient)
    Sample Mouse Cell lysate - whole cell (Bone Marrow Derived Macrophages)
    Specification Bone Marrow Derived Macrophages
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Manoj B Menon

    Verified customer

    Submitted Apr 01 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"