Overview

  • Product name
  • Description
    Goat polyclonal to SEPT7
  • Specificity
    ab79286 is expected to recognize both reported isoforms (NP_001779.3; NP_001011553.2).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide:

    C-DNNKNKGQLTKSP

    , corresponding to internal sequence amino acids 323-335 of Human SEPT7 (NP_001779.3)

  • Positive control
    • HeLa nuclear cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab79286 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    References

    ab79286 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Peritoneal macrophages)
    Permeabilization
    Yes - 0,25% Triton
    Specification
    Peritoneal macrophages
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Fixative
    Formaldehyde
    Username

    Dr. Manoj B Menon

    Verified customer

    Submitted Apr 24 2015

    Abreviews
    Application
    Western blot
    Loading amount
    10000 cells
    Gel Running Conditions
    Reduced Denaturing (7.5 to 16% gradient)
    Sample
    Mouse Cell lysate - whole cell (Bone Marrow Derived Macrophages)
    Specification
    Bone Marrow Derived Macrophages
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Manoj B Menon

    Verified customer

    Submitted Apr 01 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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