Overview

  • Product name
  • Description
    Rabbit polyclonal to Septin 11
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat
  • Immunogen

    Synthetic peptide within residues: AVAVGRPSNE ELRNLSLSGH VGFDSLPDQL VNKSTSQGFC FNILCVGETG, corresponding to N terminal amino acids 1-50 of Human Septin 11 (NP_060713).

  • Positive control
    • MCF7 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the cytoarchitecture of neurons, including dendritic arborization and dendritic spines, and in GABAergic synaptic connectivity (By similarity). During Listeria monocytogenes infection, not required for the bacterial entry process, but restricts its efficacy.
  • Tissue specificity
    Widely expressed, except in leukocytes.
  • Involvement in disease
    Note=A chromosomal aberration involving SEPT11 may be a cause of chronic neutrophilic leukemia. Translocation t(4;11)(q21;q23) with MLL/HRX.
  • Sequence similarities
    Belongs to the septin family.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell junction > synapse. Cell projection > dendritic spine. Cell projection > axon. Partly colocalizes with stress fibers and microtubules. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria.
  • Information by UniProt
  • Database links
  • Alternative names
    • SEP11_HUMAN antibody
    • SEPT 11 antibody
    • Sept11 antibody
    • Septin-11 antibody
    • Septin11 antibody
    see all

Anti-Septin 11 antibody images

  • Anti-Septin 11 antibody (ab86268) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling Septin 11 with ab86267 at 5.0µg/ml.

  • All lanes : Anti-Septin 11 antibody (ab86268) at 2 µg/ml

    Lane 1 : Human NT2 cells at 60 µg
    Lane 2 : Mouse brain extracts at 80 µg

    Secondary
    Goat anti-rabbit at 1/2000 dilution

    Predicted band size : 49 kDa
    WB Suggested Anti-Rab11a antibody (ab125456) Titration: 2 µg/ml Positive Control: Human NT-2 cells and Mouse WT brain

References for Anti-Septin 11 antibody (ab86268)

This product has been referenced in:
  • Lasagna-Reeves CA  et al. Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice. Mol Neurodegener 6:39 (2011). WB ; Mouse . Read more (PubMed: 21645391) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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