Anti-Septin 2 antibody - Aminoterminal end (ab58657)

Overview

  • Product nameAnti-Septin 2 antibody - Aminoterminal end
    See all Septin 2 primary antibodies
  • Description
    Rabbit polyclonal to Septin 2 - Aminoterminal end
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of human Septin 2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceThe septins are a family of cytoskeletal GTPases that play an essential role in cytokinesis in mammalian cells. Septin 2 (Nedd5) is a mammalian septin known to associate with actin based structures such as the contractile ring and stress fibers. It is involved in cytokinesis and may assemble into a multicomponent structure.
  • Cellular localizationCytoplasm. Cytoplasm; cytoskeleton. Cytoplasm; cytoskeleton; spindle. Chromosome; centromere; kinetochore. Cleavage furrow. Midbody. Cytoplasm; cell cortex. Cell projection; cilium membrane.
  • Database links
  • Alternative names
    • DIFF6 antibody
    • hNEDD5 antibody
    • KIAA0158 antibody
    • NEDD 5 antibody
    • NEDD5 antibody
    • Neural precursor cell expressed developmentally down regulated 5 antibody
    • Neural precursor cell expressed developmentally down regulated protein 5 antibody
    • Pnutl3 antibody
    • Protein NEDD5 antibody
    • SEPT2 antibody
    • septin 2 antibody
    see all

Anti-Septin 2 antibody - Aminoterminal end images

  • ab58657 staining Septin 2 in murine oocytes by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100 and then blocked using 1% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at a 1/25 dilution for 14 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy5®, used undiluted.

    See Abreview

References for Anti-Septin 2 antibody - Aminoterminal end (ab58657)

This product has been referenced in:
  • Zhu JL  et al. Septin2 is modified by SUMOylation and required for chromosome congression in mouse oocytes. Cell Cycle 9: (2010). WB, ICC/IF ; Mouse . Read more (PubMed: 20372094) »
  • Aminin DL  et al. Immunomodulatory effects of holothurian triterpene glycosides on mammalian splenocytes determined by mass spectrometric proteome analysis. J Proteomics 72:886-906 (2009). Read more (PubMed: 19410666) »

See all 2 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (oocyte)
Loading amount 200 cells
Specification oocyte
Gel Running Conditions Non-reduced Denaturing (10)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Oct 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (oocyte)
Specification oocyte
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% tritonX-100 in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"