Overview

  • Product name
  • Description
    Goat polyclonal to Septin 3
  • Specificity
    This antibody is expected to recognise all three human isoforms of this protein.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: SELVPEPRPKPA, corresponding to N terminal amino acids 2-13 of Human Septin 3.

  • Positive control
    • Human Brain lysate.
  • General notes
    Principal Names – SEPT3; septin 3; SEP3; bK250D10.3; neuronal-specific septin 3. Official Gene Symbol - SEPT3. GenBank Accession Number – NP_061979; NP_663786; NP_663787. LocusLink ID - 55964; 24050 (mouse); 56003 (rat).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 40 kDa.Can be blocked with Human Septin 3 peptide (ab22917).
IP Use at an assay dependent concentration. PubMed: 17564677

Target

References

This product has been referenced in:
  • Fujishima K  et al. Targeted disruption of Sept3, a heteromeric assembly partner of Sept5 and Sept7 in axons, has no effect on developing CNS neurons. J Neurochem 102:77-92 (2007). IP . Read more (PubMed: 17564677) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Jan 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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