Overview

  • Product nameAnti-Septin 3 antibody
    See all Septin 3 primary antibodies
  • Description
    Mouse monoclonal to Septin 3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 236-346 of Human Septin 3

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis.
    • Tissue specificityBrain-specific.
    • Sequence similaritiesBelongs to the septin family.
    • Post-translational
      modifications
      Phosphorylated by PKG on serine residues. Phosphorylated by PKG on Ser-91.
    • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse.
    • Information by UniProt
    • Database links
    • Alternative names
      • bK250D10.3 antibody
      • MGC133218 antibody
      • Neuronal specific septin 3 antibody
      • Neuronal-specific septin-3 antibody
      • OTTHUMP00000028742 antibody
      • SEP 3 antibody
      • SEP3 antibody
      • SEPT 3 antibody
      • sept3 antibody
      • SEPT3 protein antibody
      • SEPT3_HUMAN antibody
      • Septin3 antibody
      see all

    Anti-Septin 3 antibody images



    • Predicted band size : 40 kDa
      Septin 3 antibody (ab57478) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
    • Anti-Septin 3 antibody (ab57478) at 3 µg/ml + Lysates prepared from human Vero cells at 20000 cells

      Secondary
      HRP conjugated sheep polyclonal to mouse IgG at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 40 kDa
      Observed band size : 39 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      This image is a courtesy of Anonymous Abreview

      See Abreview

    References for Anti-Septin 3 antibody (ab57478)

    ab57478 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (vero)
    Loading amount 20000 cells
    Specification vero
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 23 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"