Overview

  • Product nameAnti-Septin 8 antibody
    See all Septin 8 primary antibodies
  • Description
    Rabbit polyclonal to Septin 8
  • Specificityab92668 detects endogenous levels of total Septin 8 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the internal region of Human Septin 8

  • Positive control
    • Extracts from HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using an epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/10000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 56 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Septin 8 antibody images

    • All lanes : Anti-Septin 8 antibody (ab92668) at 1/500 dilution

      Lane 1 : Extract from HeLa cells
      Lane 2 : Extract from HeLa cells with blocking peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 56 kDa

    References for Anti-Septin 8 antibody (ab92668)

    ab92668 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (brain)
    Loading amount 15 µg
    Specification brain
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

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    Verified customer

    Submitted Nov 23 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"