Overview

  • Product nameAnti-SERBP1 antibody
    See all SERBP1 primary antibodies
  • Description
    Mouse monoclonal to SERBP1
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-403 of Human SERBP1

Properties

Applications

Our Abpromise guarantee covers the use of ab57285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay play a role in the regulation of mRNA stability. Binds to the 3'-most 134 nt of the SERPINE1/PAI1 mRNA, a region which confers cyclic nucleotide regulation of message decay.
  • Tissue specificityExpressed at high level in the heart, skeletal muscle and kidney, and at low levels in placenta, liver and brain.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > perinuclear region. Also found in perinuclear regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGI 55 antibody
    • CHD3IP antibody
    • Chromodomain helicase DNA binding protein 3 interacting protein antibody
    • DKFZp564M2423 antibody
    • FLJ90489 antibody
    • HABP4L antibody
    • PAI 1 mRNA binding protein antibody
    • PAI RBP1 antibody
    • PAI-RBP1 antibody
    • PAI1 RNA-binding protein 1 antibody
    • PAIRB_HUMAN antibody
    • PAIRBP1 antibody
    • Plasminogen activator inhibitor 1 RNA binding protein antibody
    • Plasminogen activator inhibitor 1 RNA-binding protein antibody
    • Serbp1 antibody
    • SERPINE1 mRNA binding protein 1 antibody
    • SERPINE1 mRNA-binding protein 1 antibody
    see all

Anti-SERBP1 antibody images

  • SERBP1 antibody (ab57285) used in immunofluorescence at 10ug/ml on HeLa cells.


  • Predicted band size : 45 kDa
    SERBP1 antibody (ab57285) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HEK293 cells stained with ab57285 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57285, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-SERBP1 antibody (ab57285)

This product has been referenced in:
  • Peluso JJ  et al. Plasminogen activator inhibitor 1 RNA-binding protein interacts with progesterone receptor membrane component 1 to regulate progesterone's ability to maintain the viability of spontaneously immortalized granulosa cells and rat granulosa cells. Biol Reprod 88:20 (2013). WB, ICC/IF ; Rat . Read more (PubMed: 23242527) »
  • Heberlein KR  et al. A novel mRNA binding protein complex promotes localized plasminogen activator inhibitor-1 accumulation at the myoendothelial junction. Arterioscler Thromb Vasc Biol 32:1271-9 (2012). ICC/IF ; Human . Read more (PubMed: 22383705) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"