Anti-SERCA1 ATPase antibody [3D1H6] (ab233647)
Key features and details
- Mouse monoclonal [3D1H6] to SERCA1 ATPase
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-SERCA1 ATPase antibody [3D1H6]
See all SERCA1 ATPase primary antibodies -
Description
Mouse monoclonal [3D1H6] to SERCA1 ATPase -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey
Predicted to work with: Rat, Rabbit, Cow -
Immunogen
Recombinant fragment corresponding to Human SERCA1 ATPase aa 450-650. Expressed in E.coli.
Database link: O14983 -
Positive control
- WB: Recombinant human SERCA1 ATPase (aa 487-631) protein; SERCA1 ATPase (aa 487-631)-hIgGFc transfected HEK-293 lysate; C2C12, COS-7, HeLa, K562 and Jurkat cell lysates. Flow cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3D1H6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/500 - 1/2000. |
Flow Cyt
1/200 - 1/400. |
Target
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Function
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. -
Tissue specificity
Skeletal muscle, fast twitch muscle (type II) fibers. -
Involvement in disease
Brody myopathy -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
Developmental stage
Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle. -
Cellular localization
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 518117 Cow
- Entrez Gene: 487 Human
- Entrez Gene: 11937 Mouse
- Entrez Gene: 100037716 Rabbit
- Entrez Gene: 116601 Rat
- Omim: 108730 Human
- SwissProt: Q0VCY0 Cow
- SwissProt: O14983 Human
see all -
Alternative names
- fast twitch skeletal muscle isoform antibody
- AT2A1_HUMAN antibody
- ATP2A antibody
see all
Images
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All lanes : Anti-SERCA1 ATPase antibody [3D1H6] (ab233647) at 1/500 dilution
Lane 1 : C2C12 (mouse myoblast cell line) cell lysate.
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate.
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate.
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate.
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate. -
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells using ab233647 (green) at 1/200 dilution compared with a negetive control (red).
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All lanes : Anti-SERCA1 ATPase antibody [3D1H6] (ab233647) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : SERCA1 ATPase (aa487-631)-hIgGFc transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate. -
Anti-SERCA1 ATPase antibody [3D1H6] (ab233647) at 1/500 dilution + Recombinant human SERCA1 ATPase (aa 487-631) protein.
Expected MW is 42 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233647 has not yet been referenced specifically in any publications.