Overview

  • Product nameAnti-SERCA1 ATPase antibody
    See all SERCA1 ATPase primary antibodies
  • Description
    Rabbit polyclonal to SERCA1 ATPase
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human SERCA1 ATPase.

  • Positive control
    • This antibody gave a positive signal in Human, Mouse and Rat Skeletal Muscle tissue lysates and human skeletal muscle FFPE tissue sections

Properties

Applications

Our Abpromise guarantee covers the use of ab124501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 110 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionKey regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
  • Tissue specificitySkeletal muscle, fast twitch muscle (type II) fibers.
  • Involvement in diseaseBrody myopathy
  • Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
  • Developmental stageIsoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
  • Cellular localizationEndoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • fast twitch skeletal muscle isoform antibody
    • AT2A1_HUMAN antibody
    • ATP2A antibody
    • ATP2A1 antibody
    • ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
    • ATPase Ca++ transporting fast twitch 1 antibody
    • ATPase, Ca(2+)-transporting fast twitch 1 antibody
    • Calcium pump 1 antibody
    • Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
    • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
    • EC 3.6.3.8 antibody
    • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
    • Fast skeletal muscle SR calcium ATPase antibody
    • OTTHUMP00000162561 antibody
    • OTTHUMP00000162562 antibody
    • Sarcoendoplasmic reticulum calcium ATPase antibody
    • Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody
    • Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody
    • SERCA 1 antibody
    • SERCA1 antibody
    • SERCA1 truncated isoform, included antibody
    • SR Ca(2+) ATPase 1 antibody
    • SR Ca(2+)-ATPase 1 antibody
    see all

Anti-SERCA1 ATPase antibody images

  • All lanes : Anti-SERCA1 ATPase antibody (ab124501) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 110 kDa


    Exposure time : 30 seconds
  • IHC image of ab124501 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab124501, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-SERCA1 ATPase antibody (ab124501)

ab124501 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"