Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionKey regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Tissue specificitySkeletal muscle, fast twitch muscle (type II) fibers.
Involvement in diseaseBrody myopathy
Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
Developmental stageIsoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
ICC/IF image of ab92666 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92666, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - SERCA1 ATPase antibody (ab92666)
All lanes : Anti-SERCA1 ATPase antibody (ab92666) at 1/500 dilution
Lane 1 : NIH3T3 cell lysate Lane 2 : A549 cell lysate Lane 3 : HT29 cell lysate Lane 4 : A549 cell lysate with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 110 kDa
References for Anti-SERCA1 ATPase antibody (ab92666)
has not yet been referenced specifically in any publications.
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