Recombinant Anti-SERCA1 ATPase antibody [EPR7322] (ab129104)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7322] to SERCA1 ATPase
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SERCA1 ATPase antibody [EPR7322]
See all SERCA1 ATPase primary antibodies -
Description
Rabbit monoclonal [EPR7322] to SERCA1 ATPase -
Host species
Rabbit -
Specificity
ab129104 can not recognize SERCA1A (ATP2A1A). ab129104 recognizes SERCA1B (ATP2A1B) only. -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SERCA1 ATPase aa 950-1050. The exact sequence is proprietary.
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Positive control
- Human fetal muscle, Rat muscle and Mouse muscle lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7322 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa). |
Target
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Function
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. -
Tissue specificity
Skeletal muscle, fast twitch muscle (type II) fibers. -
Involvement in disease
Brody myopathy -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
Developmental stage
Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle. -
Cellular localization
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 487 Human
- Entrez Gene: 11937 Mouse
- Entrez Gene: 116601 Rat
- Omim: 108730 Human
- SwissProt: O14983 Human
- SwissProt: Q8R429 Mouse
- SwissProt: Q64578 Rat
- Unigene: 657344 Human
see all -
Alternative names
- fast twitch skeletal muscle isoform antibody
- AT2A1_HUMAN antibody
- ATP2A antibody
see all
Images
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All lanes : Anti-SERCA1 ATPase antibody [EPR7322] (ab129104) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate, boiled
Lane 2 : Human skeletal muscle lysate, unboiled
Lane 3 : Mouse skeletal muscle lysate, boiled
Lane 4 : Mouse skeletal muscle lysate, unboiled
Lane 5 : Rat skeletal muscle lysate, boiled
Lane 6 : Rat skeletal muscle lysate, unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 110 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
We recommend not to boil the samples after lysis to get desired WB results.
Exposure time:
Lanes 1 and 2: 180 seconds
Lanes 3 and 4: 10 seconds
Lanes 5 and 6: 20 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab129104 has been referenced in 2 publications.
- Tøien T et al. Maximal strength training-induced increase in efferent neural drive is not reflected in relative protein expression of SERCA. Eur J Appl Physiol 121:3421-3430 (2021). PubMed: 34498135
- Hook SC et al. TBC1D1 interacting proteins, VPS13A and VPS13C, regulate GLUT4 homeostasis in C2C12 myotubes. Sci Rep 10:17953 (2020). PubMed: 33087848