Overview

  • Product name
    Anti-SERCA1 ATPase antibody [VE121G9]
    See all SERCA1 ATPase primary antibodies
  • Description
    Mouse monoclonal [VE121G9] to SERCA1 ATPase
  • Specificity
    Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 1 (SERCA 1) ATPase.
  • Tested applications
    Suitable for: Inhibition Assay, ELISA, IP, WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Dog, Human, Pig, Amphibian
  • Immunogen

    Full length native protein (purified) corresponding to Rabbit SERCA1 ATPase. Purified rabbit skeletal muscle sarcoplasmic reticulum.

  • Epitope
    This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum.

Properties

Applications

Our Abpromise guarantee covers the use of ab2819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/2500. Detects a band of approximately 99 kDa (predicted molecular weight: 110 kDa).
ICC/IF 1/500.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/20.

Target

  • Function
    Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
  • Tissue specificity
    Skeletal muscle, fast twitch muscle (type II) fibers.
  • Involvement in disease
    Brody myopathy
  • Sequence similarities
    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
  • Developmental stage
    Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
  • Cellular localization
    Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • fast twitch skeletal muscle isoform antibody
    • AT2A1_HUMAN antibody
    • ATP2A antibody
    • ATP2A1 antibody
    • ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
    • ATPase Ca++ transporting fast twitch 1 antibody
    • ATPase, Ca(2+)-transporting fast twitch 1 antibody
    • Calcium pump 1 antibody
    • Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
    • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
    • EC 3.6.3.8 antibody
    • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
    • Fast skeletal muscle SR calcium ATPase antibody
    • OTTHUMP00000162561 antibody
    • OTTHUMP00000162562 antibody
    • Sarcoendoplasmic reticulum calcium ATPase antibody
    • Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody
    • Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody
    • SERCA 1 antibody
    • SERCA1 antibody
    • SERCA1 truncated isoform, included antibody
    • SR Ca(2+) ATPase 1 antibody
    • SR Ca(2+)-ATPase 1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of SERCA1 ATPase shows staining in A2058 cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with or ab2819 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse IgG secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of SERCA1 ATPase shows staining in HeLa cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with or ab2819 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse IgG secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of SERCA1 ATPase shows staining in U251 cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with or ab2819 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse IgG secondary antibody. Images were taken at 60X magnification.

  • Anti-SERCA1 ATPase antibody [VE121G9] (ab2819) at 1/500 dilution + Skeletal Muscle (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 99 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa,56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 secondsThe 99 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SERCA1 ATPase.
  • Anti-SERCA1 ATPase antibody [VE121G9] (ab2819) at 1/1000 dilution (in PBS tweeb 0.05% for 1 hour at 22°C) + Whole tissue lysate of human neck muscle. at 20 µg

    Secondary
    An HRP-conjugated sheep anti-mouse polyclonal at 1/4000 dilution
    Developed using the ECL technique

    Predicted band size : 110 kDa
    Observed band size : 110 kDa


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% milk for 16 hours at 22°C

    See Abreview

  • ab2819 staining SERCA1 ATPase in murine leg muscle by Immunohistochemistry (Frozen sections).
    Tissue was fixed in acetone, permeabilized using Triton X-100, blocked with 5% BSA for 1 hour at 22°C and then incubated with ab2819 at a 1/250 dilution for 1 hour at 22°C. The secondary used was a texas red conjugated donkey anti-mouse polyclonal used at a 1/500 dilution.

    See Abreview

  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human heart tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing SERCA1 ATPase ab2819 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing SERCA1 ATPase ab2819 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

References

This product has been referenced in:
  • Zoladz JA  et al. Mechanisms of Attenuation of Pulmonary V'O2 Slow Component in Humans after Prolonged Endurance Training. PLoS One 11:e0154135 (2016). WB . Read more (PubMed: 27104346) »
  • Riedl I  et al. AMPK?3 is dispensable for skeletal muscle hypertrophy induced by functional overload. Am J Physiol Endocrinol Metab 310:E461-72 (2016). Read more (PubMed: 26758685) »

See all 11 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (leg muscle)
Specification
leg muscle
Fixative
Acetone
Permeabilization
Yes - Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Apr 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Loading amount
100 µg
Specification
C2C12
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 09 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (C2C12)
Total protein in input
5000 µg
Specification
C2C12
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

Submitted Mar 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Loading amount
10 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing (10-20% tricine)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
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Abcam user community

Verified customer

Submitted May 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Neck Muscle)
Loading amount
20 µg
Specification
Neck Muscle
Gel Running Conditions
Reduced Denaturing (10-20% Tricine)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Feb 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - other (SR PREP)
Loading amount
10 µg
Specification
SR PREP
Gel Running Conditions
Reduced Denaturing (4-12% bis tris nupage)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Sep 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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