Recombinant Anti-SERCA1 ATPase antibody [VE121G9] (ab2819)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [VE121G9] to SERCA1 ATPase
- Suitable for: IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-SERCA1 ATPase antibody [VE121G9]
See all SERCA1 ATPase primary antibodies -
Description
Mouse monoclonal [VE121G9] to SERCA1 ATPase -
Host species
Mouse -
Specificity
Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 1 (SERCA 1) ATPase. -
Tested applications
Suitable for: IHC-Fr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length native protein (purified) corresponding to Rabbit SERCA1 ATPase. Purified rabbit skeletal muscle sarcoplasmic reticulum.
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Epitope
This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum. -
Positive control
- WB: Normal mouse and human skeletal muscle IHC-P: Normal mouse and human skeletal muscle IHC-Fr: Normal mouse and human skeletal muscle
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General notes
This product was switched from a hybridoma to a recombinant production format on 25th October 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
VE121G9 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr | (1) |
Use a concentration of 1 µg/ml.
|
WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 110 kDa).
|
IHC-P |
Use a concentration of 1 µg/ml.
|
Notes |
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IHC-Fr
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 110 kDa). |
IHC-P
Use a concentration of 1 µg/ml. |
Target
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Function
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. -
Tissue specificity
Skeletal muscle, fast twitch muscle (type II) fibers. -
Involvement in disease
Brody myopathy -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
Developmental stage
Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle. -
Cellular localization
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 487 Human
- Entrez Gene: 11937 Mouse
- Omim: 108730 Human
- SwissProt: O14983 Human
- SwissProt: Q8R429 Mouse
- Unigene: 657344 Human
- Unigene: 35134 Mouse
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Alternative names
- fast twitch skeletal muscle isoform antibody
- AT2A1_HUMAN antibody
- ATP2A antibody
see all
Images
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All lanes : Anti-SERCA1 ATPase antibody [VE121G9] (ab2819) at 1 µg/ml
Lane 1 : Human Skeletal Muscle tissue lysate
Lane 2 : Mouse Skeletal Muscle tissue lysate
Lane 3 : Human Brain tissue lysate
Lane 4 : Mouse Brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDaThis blot was produced using 3-8% Tris-Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab2819 was incubated overnight at 4°C at a 1µg/ml concentration. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibody at 1/20000 dilution for 1 hour at room temperature before imaging.
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IHC image of SERCA1 ATPase staining in a section of frozen normal mouse skeletal muscle performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab2819, 1µg/ml for 15 mins at room temperature and then ab125913, Goat anti-Mouse IgG1 at 1.5ugml was added for 15 mins at room temperature. This was detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
IHC image of SERCA1 ATPase staining in a section of frozen normal human skeletal muscle* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab2819, 1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
IHC image of SERCA1 ATPase staining in a section of formalin-fixed paraffin-embedded normal human skeletal muscle* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2819, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
IHC image of SERCA1 ATPase staining in a section of formalin-fixed paraffin-embedded normal mouse skeletal muscle performed on a Leica BOND™ system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2819, 1ug/ml, for 15 mins at room temperature and then ab125913, Goat anti-Mouse IgG1 at 1.5ugml was added for 15 mins at room temperature. This was detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Anti-SERCA1 ATPase antibody [VE121G9] (ab2819) at 1/1000 dilution (in PBS tweeb 0.05% for 1 hour at 22°C) + Whole tissue lysate of human neck muscle. at 20 µg
Secondary
An HRP-conjugated sheep anti-mouse polyclonal at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 5 minutesBlocking Step: 5% milk for 16 hours at 22°C
This image was generated from the Hybridoma version.
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Negative control image: IHC image of SERCA1 ATPase staining in a section of formalin-fixed paraffin-embedded normal mouse cerebellum performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2819, 1ug/ml, for 15 mins at room temperature and then ab125913, Goat anti-Mouse IgG1 at 1.5ugml was added for 15 mins at room temperature. This was detected using an HRP conjugated compact polymer system DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Negative control image: IHC image of SERCA1 ATPase staining in a section of formalin-fixed paraffin-embedded normal human cerebellum* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2819, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
Negative control image: IHC image of SERCA1 ATPase staining in a section of frozen normal human cerebral cortex* performed on a Leica BOND system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab2819, 1ug/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
Negative control image: IHC image of SERCA1 ATPase staining in a section of frozen normal mouse cerebral cortex performed on a Leica BOND system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab2819, 1ug/ml for 15 mins at room temperature and then ab125913, Goat anti-Mouse IgG1 at 1.5ugml was added for 15 mins at room temperature. This was detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab2819 has been referenced in 26 publications.
- Espinosa KG et al. Characterization of a novel zebrafish model of SPEG-related centronuclear myopathy. Dis Model Mech 15:N/A (2022). PubMed: 35293586
- Li Q et al. Dynamin-2 reduction rescues the skeletal myopathy of a SPEG-deficient mouse model. JCI Insight 7:N/A (2022). PubMed: 35763354
- Luo S et al. SPEG binds with desmin and its deficiency causes defects in triad and focal adhesion proteins. Hum Mol Genet 29:3882-3891 (2021). PubMed: 33355670
- Molenaar JP et al. Clinical, morphological and genetic characterization of Brody disease: an international study of 40 patients. Brain 143:452-466 (2020). PubMed: 32040565
- Jang K et al. Computational inference of cancer-specific vulnerabilities in clinical samples. Genome Biol 21:155 (2020). PubMed: 32600395