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Synthetic peptide - which represents a portion of Sarcoplasmic/ Endoplasmic Reticulum Calcium ATPase 2 (SERCA 2)encoded within exon 12.
Our Abpromise guarantee covers the use of ab3625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/200 - 1/400. PubMed: 19932743|
|IP||Use a concentration of 1 - 4 µg/ml.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 115 kDa.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Sample: Homogenate from Pig (lanes 1 and 2) or Rabbit (lane 4) aorta or lysate from cultured Rat aortic smooth muscle cells (lane 3)
Affinity purified Rabbit anti-SERCA2 (ab3625) (lanes 2, 3 and IP for lane 4) or control (ctrl) monoclonal anti-SERCA2 (lanes 1 and WB for lane 4).
Dilutions: 1:1,000 (lanes 1, 2 and 4) or 1:2,500 (lane 3)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"