Recombinant
RabMAb

Anti-SERCA2 ATPase antibody [EPR9392] (ab150435)

Overview

  • Product name
    Anti-SERCA2 ATPase antibody [EPR9392]
    See all SERCA2 ATPase primary antibodies
  • Description
    Rabbit monoclonal [EPR9392] to SERCA2 ATPase
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human SERCA2 ATPase aa 1-100 (N terminal).

  • Positive control
    • HeLa cells and cell lysate, A673 cell lysate, HepG2 cell lysate, Human kidney tissue, Human liver tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-SERCA2 ATPase antibody (Alexa Fluor® 488) [EPR9392] (ab195544)

    Anti-SERCA2 ATPase antibody (Alexa Fluor® 647) [EPR9392] (ab195546)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab150435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000 - 1/100000. Predicted molecular weight: 115 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle.
    • Tissue specificity
      Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart.
    • Involvement in disease
      Defects in ATP2A2 are a cause of acrokeratosis verruciformis (AKV) [MIM:101900]; also known as Hopf disease. AKV is a localized disorder of keratinization, which is inherited as an autosomal dominant trait. Its onset is early in life with multiple flat-topped, flesh-colored papules on the hands and feet, punctate keratoses on the palms and soles, with varying degrees of nail involvement. The histopathology shows a distinctive pattern of epidermal features with hyperkeratosis, hypergranulosis, and acanthosis together with papillomatosis. These changes are frequently associated with circumscribed elevations of the epidermis that are said to resemble church spires. There are no features of dyskeratosis or acantholysis, the typical findings in lesions of Darier disease.
      Defects in ATP2A2 are the cause of Darier disease (DD) [MIM:124200]; also known as Darier-White disease (DAR). DD is an autosomal dominantly inherited skin disorder characterized by loss of adhesion between epidermal cells (acantholysis) and abnormal keratinization. Patients with mild disease may have no more than a few scattered keratotic papules or subtle nail changes, whereas those with severe disease are handicapped by widespread malodorous keratotic plaques. In a few families, neuropsychiatric abnormalities such as mild mental retardation, schizophrenia, bipolar disorder and epilepsy have been reported. Stress, UV exposure, heat, sweat, friction, and oral contraception exacerbate disease symptoms. Prevalence has been estimated at 1 in 50000. Clinical variants of DD include hypertrophic, vesicobullous, hypopigmented, cornifying, zosteriform or linear, acute and comedonal subtypes. Comedonal Darier disease (CDD) is characterized by the coexistence of acne-like comedonal lesions with typical Darier hyperkeratotic papules on light-exposed areas. At histopathologic level, CDD differs from classic DD in the prominent follicular involvement and the presence of greatly elongated dermal villi.
    • Sequence similarities
      Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
    • Post-translational
      modifications
      Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity.
    • Cellular localization
      Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AT2A2_HUMAN antibody
      • Atp2a2 antibody
      • ATP2B antibody
      • ATPase Ca++ transporting cardiac muscle slow twitch 2 antibody
      • Calcium pump 2 antibody
      • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
      • Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform antibody
      • Cardiac Ca2+ ATPase antibody
      • DAR antibody
      • DD antibody
      • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
      • MGC45367 antibody
      • Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 antibody
      • SERCA 2 antibody
      • SERCA2 antibody
      • serca2a antibody
      • slow twitch skeletal muscle isoform antibody
      • SR Ca(2+)-ATPase 2 antibody
      see all

    Images

    • Immunofluorescence analysis of HeLa cells labelling SERCA2 ATPase with ab150435 at 1/100.
    • Immunohistochemical analysis of paraffin embedded Human liver tissue labelling SERCA2 ATPase with ab150435 at 1/50.
    • Overlay histogram showing HepG2 cells stained with ab150435 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150435, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-SERCA2 ATPase antibody [EPR9392] (ab150435) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A673 cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP labelled antibody at 1/2000 dilution

      Predicted band size : 115 kDa
    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling SERCA2 ATPase with ab150435 at 1/50.

    References

    This product has been referenced in:
    • Löser S  et al. Pulmonary ORMDL3 is critical for induction of Alternaria-induced allergic airways disease. J Allergy Clin Immunol 139:1496-1507.e3 (2017). WB ; Human . Read more (PubMed: 27623174) »
    • Wu J  et al. Gp130-mediated STAT3 activation by S-propargyl-cysteine, an endogenous hydrogen sulfide initiator, prevents doxorubicin-induced cardiotoxicity. Cell Death Dis 7:e2339 (2016). Read more (PubMed: 27537522) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (Cardiomyocytes)
    Permeabilization
    Yes - Triton x-100, 0.01%
    Specification
    Cardiomyocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 15 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up