Anti-SERCA3 ATPase antibody (ab54876)

Overview

  • Product name
    Anti-SERCA3 ATPase antibody
    See all SERCA3 ATPase primary antibodies
  • Description
    Mouse monoclonal to SERCA3 ATPase
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cyt, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 501-621 of Human SERCA3 ATPase

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab54876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 114 kDa.
IHC-P Use a concentration of 3 µg/ml.
IP Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.

Target

  • Function
    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
  • Tissue specificity
    Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues.
  • Sequence similarities
    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
  • Cellular localization
    Nucleus membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosine triphosphatase calcium antibody
    • AT2A3_HUMAN antibody
    • Atp2a3 antibody
    • ATPase Ca(2+) transporting ubiquitous antibody
    • ATPase Ca++ transporting ubiquitous antibody
    • Calcium pump 3 antibody
    • Calcium translocating P type ATPase antibody
    • Sarco/endoplasmic reticulum Ca2+ ATPase antibody
    • Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 antibody
    • SERCA 3 antibody
    • SERCA3 antibody
    • SERCA3b antibody
    • SR Ca(2+) ATPase 3 antibody
    • SR Ca(2+)-ATPase 3 antibody
    see all

Images

  • Overlay histogram showing Jurkat cells stained with ab54876 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54876, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • SERCA3 ATPase antibody (ab54876) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 114 kDa
    SERCA3 ATPase antibody (ab54876) at 1ug/lane + HL-60 cell lysate at 25ug/lane.
  • ab54876 staining SERCA3 ATPase in jurkat cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100 and then blocked using 3% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 23°C. The secondary antibody used was a chicken anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution. Nuclei stained with Hoechst 33342.

    See Abreview

References

ab54876 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (C2C12)
Total protein in input
3000 µg
Specification
C2C12
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

Submitted Mar 09 2012

Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Loading amount
100 µg
Specification
C2C12
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Mar 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat T lymphocytes)
Loading amount
50 µg
Specification
Jurkat T lymphocytes
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3%
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Verified customer

Submitted Feb 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (Jurkat)
Specification
Jurkat
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 31 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X
Username

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Verified customer

Submitted Aug 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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