Anti-SERCA3 ATPase antibody (ab54876)

Overview

  • Product nameAnti-SERCA3 ATPase antibody
    See all SERCA3 ATPase primary antibodies
  • Description
    Mouse monoclonal to SERCA3 ATPase
  • Tested applicationsSuitable for: WB, IHC-P, IP, Flow Cyt, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 501-621 of Human SERCA3 ATPase

Properties

Applications

Our Abpromise guarantee covers the use of ab54876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 114 kDa.
IHC-P Use a concentration of 3 µg/ml.
IP Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.

Target

  • FunctionThis magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
  • Tissue specificityFound in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues.
  • Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
  • Cellular localizationNucleus membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosine triphosphatase calcium antibody
    • AT2A3_HUMAN antibody
    • Atp2a3 antibody
    • ATPase Ca(2+) transporting ubiquitous antibody
    • ATPase Ca++ transporting ubiquitous antibody
    • Calcium pump 3 antibody
    • Calcium translocating P type ATPase antibody
    • Sarco/endoplasmic reticulum Ca2+ ATPase antibody
    • Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 antibody
    • SERCA 3 antibody
    • SERCA3 antibody
    • SERCA3b antibody
    • SR Ca(2+) ATPase 3 antibody
    • SR Ca(2+)-ATPase 3 antibody
    see all

Anti-SERCA3 ATPase antibody images

  • Overlay histogram showing Jurkat cells stained with ab54876 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54876, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • SERCA3 ATPase antibody (ab54876) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 114 kDa
    SERCA3 ATPase antibody (ab54876) at 1ug/lane + HL-60 cell lysate at 25ug/lane.
  • ab54876 staining SERCA3 ATPase in jurkat cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100 and then blocked using 3% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 23°C. The secondary antibody used was a chicken anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution. Nuclei stained with Hoechst 33342.

    See Abreview

References for Anti-SERCA3 ATPase antibody (ab54876)

ab54876 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (C2C12)
Total protein in input 3000 µg
Specification C2C12
Immuno-precipitation step Protein A
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Submitted Mar 09 2012

Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12)
Loading amount 100 µg
Specification C2C12
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Mar 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat T lymphocytes)
Loading amount 50 µg
Specification Jurkat T lymphocytes
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3%
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Submitted Feb 01 2011

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Application Immunocytochemistry
Sample Human Cultured Cells (Jurkat)
Specification Jurkat
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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Submitted Jan 31 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X
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Submitted Aug 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"