Anti-SERCA3 ATPase antibody - N-terminal (ab154259)
Key features and details
- Rabbit polyclonal to SERCA3 ATPase - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SERCA3 ATPase antibody - N-terminal -
Description
Rabbit polyclonal to SERCA3 ATPase - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment, corresponding to a region within N -terminal amino acids 3-185 of Human SERCA3 ATPase (Uniprot ID: Q93084).
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Positive control
- Jurkat, Raji, K562 and BCL1 whole cell lysates; Human colon carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 114 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 114 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. -
Tissue specificity
Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
Cellular localization
Nucleus membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 395707 Chicken
- Entrez Gene: 512313 Cow
- Entrez Gene: 489 Human
- Entrez Gene: 53313 Mouse
- Entrez Gene: 25391 Rat
- Entrez Gene: 495440 Xenopus laevis
- Entrez Gene: 779786 Xenopus tropicalis
- Omim: 601929 Human
see all -
Alternative names
- Adenosine triphosphatase calcium antibody
- AT2A3_HUMAN antibody
- Atp2a3 antibody
see all
Images
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All lanes : Anti-SERCA3 ATPase antibody - N-terminal (ab154259) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : K562 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 114 kDa
5% SDS PAGE -
Anti-SERCA3 ATPase antibody - N-terminal (ab154259) at 1/5000 dilution + BCL1 whole cell lysate at 30 µg
Predicted band size: 114 kDa
5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling SERCA3 ATPase with ab154259 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154259 has been referenced in 1 publication.
- Guo X et al. Enhanced store-operated calcium entry in platelets is associated with acute coronary syndrome. Acta Biochim Biophys Sin (Shanghai) 52:207-210 (2020). PubMed: 31942931