Overview

  • Product nameAnti-Serglycin antibody
    See all Serglycin primary antibodies
  • Description
    Mouse monoclonal to Serglycin
  • Tested applicationsSuitable for: ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length SRGN protein (AAH15516, 1 a.a. ~ 159 a.a) with a 26 kDa tag

  • Positive control
    • Recombinant full length SRGN protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab76512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 18 kDa.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPlays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF-alpha and may also regulate protease secretion. Inhibits bone mineralization.
  • Sequence similaritiesBelongs to the serglycin family.
  • Post-translational
    modifications
    O-glcosylated; contains chondroitin sulfate and heparan sulfate.
  • Cellular localizationCytoplasmic granule. Secreted > extracellular space. Golgi apparatus. Found in mast cell granules and in cytoplasmic granules of cytolytic T lymphocytes from where it is secreted upon cell activation. Secreted constitutively by endothelial cells and macrophages. Located to Golgi apparatus during neutrophil differentiation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chondroitin sulfate proteoglycan core protein antibody
    • Cytolytic granule proteoglycan core protein antibody
    • FLJ12930 antibody
    • gp600 antibody
    • Hematopoetic proteoglycan core protein antibody
    • Mastocytoma proteoglycan core protein antibody
    • MGC9289 antibody
    • OTTHUMP00000019716 antibody
    • P.PG antibody
    • PG19 core protein antibody
    • Pgsg antibody
    • Platelet proteoglycan core protein antibody
    • platelet proteoglycan protein core antibody
    • PLATELET PROTEOGLYCAN PROTEIN CORE; PPG antibody
    • PPG antibody
    • PRG antibody
    • PRG1 antibody
    • PROTEOGLYCAN 1 antibody
    • proteoglycan 1, secretory granule antibody
    • Proteoglycan 10K core protein antibody
    • Proteoglycan peptide core protein antibody
    • PROTEOGLYCAN PROTEIN CORE FOR MAST CELL SECRETORY GRANULE antibody
    • secretory granule proteoglycan 1 antibody
    • Secretory granule proteoglycan core peptide antibody
    • Secretory granule proteoglycan core protein antibody
    • Serglycin antibody
    • serglycin proteoglycan antibody
    • Sgc antibody
    • Srgn antibody
    • SRGN_HUMAN antibody
    see all

Anti-Serglycin antibody images

  • Overlay histogram showing Jurkat cells stained with ab76512 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76512, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-Serglycin antibody (ab76512) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 18 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Note: Molecular weight of the tag alone is 26 kDa.

References for Anti-Serglycin antibody (ab76512)

ab76512 has not yet been referenced specifically in any publications.

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