Anti-Serine Palmitoyltransferase antibody (ab23696)

Overview

  • Product name
    Anti-Serine Palmitoyltransferase antibody
    See all Serine Palmitoyltransferase primary antibodies
  • Description
    Rabbit polyclonal to Serine Palmitoyltransferase
  • Specificity
    This antibody detects human serine palmitoyltransferase subunit SPT2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Chicken, Cow, Human, Pig, Xenopus laevis, Zebrafish, African green monkey
  • Immunogen

    Synthetic peptide:

    LDRPFDETTYEETED

    , corresponding to amino acids 548-562 of Human Serine Palmitoyltransferase.

  • Positive control
    • Mouse brain homogenate, rat kidney supernatant, human liver microsome.

Properties

Applications

Our Abpromise guarantee covers the use of ab23696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function
    Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SSSPTA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SSSPTB complex displays a preference for C18-CoA substrate.
  • Tissue specificity
    Widely expressed.
  • Pathway
    Lipid metabolism; sphingolipid metabolism.
  • Involvement in disease
    Defects in SPTLC2 are the cause of hereditary sensory and autonomic neuropathy type 1C (HSAN1C) [MIM:613640]. It is a form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by prominent sensory abnormalities with a variable degree of motor and autonomic dysfunction. The neurological phenotype is often complicated by severe infections, osteomyelitis, and amputations. HSAN1C symptoms include loss of touch and vibration in the feet, dysesthesia and severe panmodal sensory loss in the upper and lower limbs, distal lower limb sensory loss with ulceration and osteomyelitis, and distal muscle weakness.
  • Sequence similarities
    Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0526 antibody
    • LCB 2 antibody
    • LCB2 antibody
    • LCB2a antibody
    • Long chain base biosynthesis protein 2 antibody
    • Long chain base biosynthesis protein 2a antibody
    • Serine palmitoyl CoA transferase 2 antibody
    • Serine palmitoyltransferase 2 antibody
    • Serine palmitoyltransferase long chain base subunit 2 antibody
    • Serine palmitoyltransferase subunit II antibody
    • Serine-palmitoyl-CoA transferase 2 antibody
    • SPT 2 antibody
    • SPT2 antibody
    • SPTC2_HUMAN antibody
    • SPTLC 2 antibody
    • Sptlc2 antibody
    see all

Anti-Serine Palmitoyltransferase antibody images

  • All lanes : Anti-Serine Palmitoyltransferase antibody (ab23696) at 1 µg/ml

    Lane 1 : 30ug mouse brain homogenate.
    Lane 2 : 30ug rat kidney supernatant.
    Lane 3 : 30ug human liver microsome.


    Predicted band size : 62 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)

References for Anti-Serine Palmitoyltransferase antibody (ab23696)

This product has been referenced in:
  • Martínez-Sánchez N  et al. Hypothalamic AMPK-ER Stress-JNK1 Axis Mediates the Central Actions of Thyroid Hormones on Energy Balance. Cell Metab 26:212-229.e12 (2017). WB ; Mouse, Rat . Read more (PubMed: 28683288) »
  • Kawamoto E  et al. Immobilization rapidly induces muscle insulin resistance together with the activation of MAPKs (JNK and p38) and impairment of AS160 phosphorylation. Physiol Rep 4:N/A (2016). Rat . Read more (PubMed: 27482072) »

See all 9 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, low pH
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 25 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (whole)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Treatment
500nM NE
Specification
whole
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 20 2017

Thank you for contacting us.

Here are the testing discount codes as requested:

Anti-IDH3G antibody (ab118289) Discount code: 42379

Anti-Serine Palmitoyltransferase antibody (ab23696): this antibody has already been shown t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount
50 µg
Specification
Mouse embryonic fibroblast
Gel Running Conditions
Reduced Denaturing (10-20% tris-tricine)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up