Anti-Serine Palmitoyltransferase antibody (ab23696)
Key features and details
- Rabbit polyclonal to Serine Palmitoyltransferase
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Serine Palmitoyltransferase antibody
See all Serine Palmitoyltransferase primary antibodies -
Description
Rabbit polyclonal to Serine Palmitoyltransferase -
Host species
Rabbit -
Specificity
This antibody detects human serine palmitoyltransferase subunit SPT2 -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide:
LDRPFDETTYEETED
, corresponding to amino acids 548-562 of Human Serine Palmitoyltransferase. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purifed. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. |
Target
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Function
Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SSSPTA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SSSPTB complex displays a preference for C18-CoA substrate. -
Tissue specificity
Widely expressed. -
Pathway
Lipid metabolism; sphingolipid metabolism. -
Involvement in disease
Defects in SPTLC2 are the cause of hereditary sensory and autonomic neuropathy type 1C (HSAN1C) [MIM:613640]. It is a form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by prominent sensory abnormalities with a variable degree of motor and autonomic dysfunction. The neurological phenotype is often complicated by severe infections, osteomyelitis, and amputations. HSAN1C symptoms include loss of touch and vibration in the feet, dysesthesia and severe panmodal sensory loss in the upper and lower limbs, distal lower limb sensory loss with ulceration and osteomyelitis, and distal muscle weakness. -
Sequence similarities
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 9517 Human
- Entrez Gene: 20773 Mouse
- Entrez Gene: 366697 Rat
- Omim: 605713 Human
- SwissProt: O15270 Human
- SwissProt: P97363 Mouse
- Unigene: 435661 Human
- Unigene: 565 Mouse
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Alternative names
- KIAA0526 antibody
- LCB 2 antibody
- LCB2 antibody
see all
Images
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All lanes : Anti-Serine Palmitoyltransferase antibody (ab23696) at 1 µg/ml
Lane 1 : 30ug mouse brain homogenate.
Lane 2 : 30ug rat kidney supernatant.
Lane 3 : 30ug human liver microsome.
Predicted band size: 62 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab23696 has been referenced in 25 publications.
- Takeda S et al. Comparative Study on Epidermal Moisturizing Effects and Hydration Mechanisms of Rice-Derived Glucosylceramides and Ceramides. Int J Mol Sci 24:N/A (2022). PubMed: 36613524
- Hwang S et al. Consequences of aneuploidy in human fibroblasts with trisomy 21. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33526671
- Takeda S et al. ß-Sitosterol 3-O-D-glucoside increases ceramide levels in the stratum corneum via the up-regulated expression of ceramide synthase-3 and glucosylceramide synthase in a reconstructed human epidermal keratinization model. PLoS One 16:e0248150 (2021). PubMed: 33684145
- Zhou Q et al. Effect of Gegen Qinlian Decoction on Hepatic Gluconeogenesis in ZDF Rats with Type 2 Diabetes Mellitus Based on the Farnesol X Receptor/Ceramide Signaling Pathway Regulating Mitochondrial Metabolism and Endoplasmic Reticulum Stress. Evid Based Complement Alternat Med 2021:9922292 (2021). PubMed: 34422083
- Bugajev V et al. Crosstalk between ORMDL3, serine palmitoyltransferase, and 5-lipoxygenase in the sphingolipid and eicosanoid metabolic pathways. J Lipid Res 62:100121 (2021). PubMed: 34560079