The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
Abcam recommends blocking with 3% Milk.
FunctionCatalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.
Tissue specificityBrain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver.
Sequence similaritiesBelongs to the serine/threonine dehydratase family.
Post-translational modificationsS-nitrosylated, leading to decrease the enzyme activity.