• Product nameAnti-Serine racemase antibody
    See all Serine racemase primary antibodies
  • Description
    Rabbit polyclonal to Serine racemase
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity

    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Serine racemase.

    (Peptide available as ab45543.)

  • Positive control
    • This antibody gave a positive signal in Human and Mouse Brain tissue lysate as well as SHSY-5Y whole cell lysate.


Associated products


Our Abpromise guarantee covers the use of ab45434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36.5 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionCatalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.
  • Tissue specificityBrain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver.
  • Sequence similaritiesBelongs to the serine/threonine dehydratase family.
  • Post-translational
    S-nitrosylated, leading to decrease the enzyme activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • D serine ammonia lyase antibody
    • D serine dehydratase antibody
    • D-serine ammonia-lyase antibody
    • D-serine dehydratase antibody
    • ILV1 antibody
    • ISO1 antibody
    • L serine ammonia lyase antibody
    • L serine dehydratase antibody
    • L-serine ammonia-lyase antibody
    • L-serine dehydratase antibody
    • Serine racemase antibody
    • srr antibody
    • SRR_HUMAN antibody
    see all

Anti-Serine racemase antibody images

  • ICC/IF image of ab45434 stained human SHSY5Y cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45434, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red).  DAPI was used to stain the cell nuclei (blue). However, this Fast-Track antibody is not yet fully characterised.  This image represents inconclusive preliminary data.

  • All lanes : Anti-Serine racemase antibody (ab45434) at 1 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : Human brain tissue lysate - total protein (ab29466)
    Lane 3 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 36.5 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,55 kDa,98 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Serine racemase antibody (ab45434)

This product has been referenced in:
  • Otte DM  et al. Effects of Chronic D-Serine Elevation on Animal Models of Depression and Anxiety-Related Behavior. PLoS One 8:e67131 (2013). Mouse . Read more (PubMed: 23805296) »
  • Singh NS  et al. Nicotinic acetylcholine receptor antagonists alter the function and expression of serine racemase in PC-12 and 1321N1 cells. Cell Signal 25:2634-45 (2013). Read more (PubMed: 24012499) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spinal cord)
Permeabilization No
Specification Spinal cord
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde

Mr. Yahyah Aman

Verified customer

Submitted Jun 24 2015