The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36.5 kDa).
Use a concentration of 5 µg/ml.
FunctionCatalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.
Tissue specificityBrain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver.
Sequence similaritiesBelongs to the serine/threonine dehydratase family.
Post-translational modificationsS-nitrosylated, leading to decrease the enzyme activity.
ICC/IF image of ab45434 stained human SHSY5Y cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45434, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). However, this Fast-Track antibody is not yet fully characterised.This image represents inconclusive preliminary data.
Western blot - Serine racemase antibody (ab45434)
All lanes : Anti-Serine racemase antibody (ab45434) at 1 µg/ml
Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 2 : Human brain tissue lysate - total protein (ab29466) Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
References for Anti-Serine racemase antibody (ab45434)
This product has been referenced in:
Otte DM et al. Effects of Chronic D-Serine Elevation on Animal Models of Depression and Anxiety-Related Behavior. PLoS One8:e67131 (2013).
Read more (PubMed: 23805296) »
Singh NS et al. Nicotinic acetylcholine receptor antagonists alter the function and expression of serine racemase in PC-12 and 1321N1 cells. Cell Signal25:2634-45 (2013).
Read more (PubMed: 24012499) »