Overview

  • Product name
    Anti-Serine/threonine-protein kinase 4 antibody
    See all Serine/threonine-protein kinase 4 primary antibodies
  • Description
    Rabbit polyclonal to Serine/threonine-protein kinase 4
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Serine/threonine-protein kinase 4 aa 324-388.
    Sequence:

    EMDSGTMVRAVGDEMGTVRVASTMTDGANTMIEHDDTLPSQLGTMVINAE DEEEEGTMKRRDETM

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4, HeLa or Raji cells. Cal27 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab97399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SARAH domain.
  • Post-translational
    modifications
    Autophosphorylated on serine and threonine residues.
  • Cellular localization
    Cytoplasm. Nucleus. The caspase-cleaved form cycles between the nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kinase responsive to stress antibody
    • Krs2 antibody
    • Mammalian STE20 like protein kinase 1 antibody
    • Mammalian STE20-like protein kinase 1 antibody
    • Mammalian sterile 20 like 1 antibody
    • MST-1 antibody
    • MST1 antibody
    • Serine/threonine kinase 4 antibody
    • Serine/threonine protein kinase Krs 2 antibody
    • Serine/threonine-protein kinase 4 antibody
    • Serine/threonine-protein kinase Krs-2 antibody
    • STE20 like kinase MST1 antibody
    • STE20-like kinase MST1 antibody
    • STK4 antibody
    • STK4_HUMAN antibody
    • TIIAC antibody
    • YSK3 antibody
    see all

Images

  • Anti-Serine/threonine-protein kinase 4 antibody (ab97399) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 56 kDa
    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab97399 at 1/500 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97399 at 1/200 dilution (top image) and merged with DNA probe (bottom image).

References

This product has been referenced in:
  • Meliopoulos VA  et al. MicroRNA regulation of human protease genes essential for influenza virus replication. PLoS One 7:e37169 (2012). WB ; Human . Read more (PubMed: 22606348) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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