Anti-Serotonin antibody (ab6444)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Serotonin
  • Specificity
    Using a conjugate 5-Hydroxytryptamine-(protein carriers), antibody specificity was performed with an ELISA test by competition experiments with the following compounds
    CompoundCross-reactivity ratio (a)
    Serotonin-G-(Pc)1
    5-Methoxytryptamine-G-(Pc)1/25
    Tryptamine-G-(Pc)1/50
    5-Hydroxytryptophan-G-(Pc)1/175
    L-Tryptophan-G-(Pc)1/3500
    5-Methoxytryptophan-G-(Pc)1/20000
    free Serotonin 1/115
    (a) : 5-Hydroxytryptamine-G-(Pc) concentration / Other c conjugated indolealkylamines concentration at half displacement ; G = Glutaraldehyde
  • Tested applications
    Suitable for: ELISA, ICCmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Serotonin. Synthetic 5-Hydroxytryptamine conjugated to protein carrier (PC)

  • General notes

    ab6444 specific to conjugated 5-Hydroxytryptamine (Serotonin)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • Relevance
    Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.
  • Cellular localization
    Cytoplasmic and Secreted
  • Alternative names
    • 5 hydroxytryptamine antibody
    • 5HT antibody

References for Anti-Serotonin antibody (ab6444)

ab6444 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Permeabilization
No
Specification
Colon
Blocking step
hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde
Username

Vinicius Kannen Cardoso

Verified customer

Submitted Aug 10 2016

Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

There is a mistake on the datasheet, and the antibody was raised against serotonin conjugated to a protein carrier atthe a...

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