Overview

  • Product nameAnti-Serotonin antibody
    See all Serotonin primary antibodies
  • Description
    Goat polyclonal to Serotonin
  • Tested applicationsSuitable for: IHC-Fr, IHC-FoFr, ICC/IFmore details
  • Immunogen

    Serotonin whole molecule conjugated to BSA with paraformaldehyde.

  • Positive control
    • Rat hypothalamus, raphe nuclei and spinal cord.
  • General notesUpon delivery dilute with phosphate buffer or Tris buffer at dilutions no higher than 1/10, aliquot and freeze at -15° C or lower. Antibody can be stored for up to six months if handled as described above.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab66047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr 1/400 - 1/800. When using Biotin/Streptavidin-HRP Technique dilution of 1/5000 - 1/10000.
ICC/IF Use at an assay dependent concentration. PubMed: 22761438

Target

  • RelevanceSerotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.
  • Cellular localizationCytoplasmic and Secreted
  • Alternative names
    • 5 hydroxytryptamine antibody
    • 5HT antibody

Anti-Serotonin antibody images

  • ab66407 staining of rat hypothalamus using 4+ biotin-streptavidin/HRP staing at 1/4000.
  • ab66047, staining Serotonin in RBL-2H3 rat mast cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100 and blocked with 3% BSA for 60 min. Samples were incubated with primary antibody (1/500 dilution in TBS + 100 mm Tris-HCl + 150 mm NaCl). An AlexaFluor®647-conjugated anti-goat IgG was used as the secondary antibody (1/500).
  • ab66047 staining serotonin in SKNSH cells treated with fluoxetine hydrochloride (ab120077), by ICC/IF. Increase of serotonin expression correlates with increased concentration of fluoxetine hydrochloride, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120077 (fluoxetine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66047 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 donkey anti-goat polyclonal antibody (ab96931) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References for Anti-Serotonin antibody (ab66047)

This product has been referenced in:
  • Steinfeld R  et al. Divergent innervation of the olfactory bulb by distinct raphe nuclei. J Comp Neurol 523:805-13 (2015). IHC (PFA fixed) . Read more (PubMed: 25420775) »
  • Moreno-Sanchez D  et al. Polyphosphate Is a Novel Pro-inflammatory Regulator of Mast Cells and Is Located in Acidocalcisomes. J Biol Chem 287:28435-44 (2012). ICC/IF . Read more (PubMed: 22761438) »

See all 3 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
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Submitted Nov 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"