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Serotonin (conjugated) antibody (ab8882)

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab8882 for help.

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2 questions for ab8882

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Question 1

Friday 25-May-2012

Inquiry: I would like to order two antibodies, but would first like to check what the antigen was conjugated to. If it is KLH, then I will not be able to use them. Can you tell me what the antigens for ab37093 (anti-tyramine) and ab8882 (anti-5HT) were conjugated to? Thank you kindly,

ANSWER:

 

Thank you for contacting us with your question.
The immunogens for both of these antibodies were conjugated to glutaraldehyde and BSA, not KLH, so they should be appropriate for your studies.
I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you.

Question 2

Friday 09-September-2011

I am interested to use the antibody to detect serotonin in an ELISA assay. Is there a protocol for this and what is the detection sensitivity ? Thank you

ANSWER:

 

Thank you for your interest in Abcam's Anti-Serotonin antibodies.

I have performed a search of our catalog and I would like to recommend ab8882. This is an anti-serotonin antibody that has been validated for ELISA.

There is no specific protocol that is recommended, however we do recommend a starting dilution of 1/2000 - 1/5000.

I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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