The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and Phospho-Thr29 AA-NAT, catalog ab3439. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~ 70-fold) for competitive inhibition of antibody-protein binding reactions.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Arylalkylamine N acetyltransferase
FunctionControls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
Tissue specificityHighly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.
PathwayAromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.
Involvement in diseaseDelayed sleep phase syndrome
Sequence similaritiesBelongs to the acetyltransferase family. AANAT subfamily. Contains 1 N-acetyltransferase domain.
Post-translational modificationscAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.