Purification notesTotal anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide.
Phospho-specific N-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.
Primary antibody notesPineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.
Western blot - Serotonin N-acetyltransferase (phospho T29) antibody (ab3439)
Predicted band size : 23 kDa
Western blot detection of Serotonin N-acetyltransferase (phospho T29) in transfected 293T cell lysate using ab3439.
References for Anti-Serotonin N-acetyltransferase (phospho T29) antibody (ab3439)
This product has been referenced in:
Hou B et al. Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS Rats. Front Cell Neurosci11:98 (2017).
IHC (PFA fixed)
Read more (PubMed: 28473754) »