Anti-Serotonin N-acetyltransferase (phospho T29) antibody (ab3439)

Overview

  • Product name
    Anti-Serotonin N-acetyltransferase (phospho T29) antibody
    See all Serotonin N-acetyltransferase primary antibodies
  • Description
    Rabbit polyclonal to Serotonin N-acetyltransferase (phospho T29)
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Chicken, Hamster, Human, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse Serotonin N-acetyltransferase aa 24-33 (N terminal) (phospho T29).
    Sequence: CQRRHTpLPAS
    (Peptide available as ab4975)

  • Positive control
    • Rat pineal gland whole protein extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific N-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.
  • Primary antibody notes
    Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).Can be blocked with Serotonin N-acetyltransferase peptide (ab4975).

By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract.

Target

  • Function
    Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
  • Tissue specificity
    Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.
  • Pathway
    Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.
  • Involvement in disease
    Delayed sleep phase syndrome
  • Sequence similarities
    Belongs to the acetyltransferase family. AANAT subfamily.
    Contains 1 N-acetyltransferase domain.
  • Post-translational
    modifications
    cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA-NAT antibody
    • Aanat antibody
    • Aralkylamine N-acetyltransferase antibody
    • Arylalkylamine N acetyltransferase antibody
    • Serotonin acetylase antibody
    • Serotonin N-acetyltransferase antibody
    • SNAT antibody
    • SNAT_HUMAN antibody
    see all

Images



  • Predicted band size : 23 kDa

    Western blot detection of Serotonin N-acetyltransferase (phospho T29) in transfected 293T cell lysate using ab3439.

References

This product has been referenced in:
  • Hou B  et al. Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS Rats. Front Cell Neurosci 11:98 (2017). IHC (PFA fixed) ; Rat . Read more (PubMed: 28473754) »
  • Moran AW  et al. Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners. Br J Nutr 104:637-46 (2010). Read more (PubMed: 20338074) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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