Highly pure (>98%) recombinant full length human SERPINA12.
FormLyophilised:Reconstitute in 500 µl sterile PBS containing 0.1% BSA to a concentration of 0.1 mg/ml.
SDS states: Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. [Checker:I have entered 0.1 mg/ml on Details page for porotein conc.]
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: (Direct or Sandwich) Use at a concentration of 0.25 - 1 µg/ml, at 100µl per well.
This allows the detection of at least 0.2 - 0.4 ng/well of recombinant SERPINA12.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 46 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant SERPINA12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay modulates insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues.
Tissue specificityExpressed in visceral adipose tissues.
Sequence similaritiesBelongs to the serpin family.