Overview

  • Product nameAnti-SerpinB2 antibody
    See all SerpinB2 primary antibodies
  • Description
    Rabbit polyclonal to SerpinB2
  • SpecificityThis antibody is specific for SerpinB2 (but not the shorter form).
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide based on the aminoterminal end of mature human SerpinB2

Properties

Applications

Our Abpromise guarantee covers the use of ab47742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notesIHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 46 kDa. Dilution optimised using Chromogenic detection.

    Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.
    • Sequence similaritiesBelongs to the serpin family. Ov-serpin subfamily.
    • Post-translational
      modifications
      The signal sequence is not cleaved.
    • Cellular localizationCytoplasm. Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • HsT1201 antibody
      • Monocyte Arg serpin antibody
      • Monocyte Arg-serpin antibody
      • Monocyte Arginine-serpin antibody
      • Monocyte-derived plasminogen activator inhibitor antibody
      • PAI antibody
      • PAI-2 antibody
      • PAI2 antibody
      • PAI2_HUMAN antibody
      • Placental plasminogen activator inhibitor antibody
      • PLANH2 antibody
      • Plasminogen activator inhibitor 2 antibody
      • plasminogen activator inhibitor, type 2 antibody
      • plasminogen activator inhibitor, type II (arginine-serpin) antibody
      • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2 antibody
      • Serpin B2 antibody
      • serpin peptidase inhibitor, clade B (ovalbumin), member 2 antibody
      • Serpinb2 antibody
      • Urokinase inhibitor antibody
      see all

    Anti-SerpinB2 antibody images

    • Ab47742 staining human normal temporal lobe. Staining is localized to the cytoplasm.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Anti-SerpinB2 antibody (ab47742) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 46 kDa
      Observed band size : 49 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute

    References for Anti-SerpinB2 antibody (ab47742)

    This product has been referenced in:
    • Peng H  et al. microRNA-31/factor-inhibiting hypoxia-inducible factor 1 nexus regulates keratinocyte differentiation. Proc Natl Acad Sci U S A 109:14030-4 (2012). WB, IHC-P ; Human . Read more (PubMed: 22891326) »
    • Angelucci S  et al. Proteome analysis of human Wharton's jelly cells during in vitro expansion. Proteome Sci 8:18 (2010). WB ; Human . Read more (PubMed: 20346146) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 100000 cells
    Gel Running Conditions Reduced Denaturing (4-20% tris-Glycin gel)
    Sample Human Cell lysate - whole cell (Bxpc3)
    Specification Bxpc3
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Dec 12 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"