Overview

  • Product name
  • Description
    Rabbit polyclonal to SerpinB2
  • Specificity
    This antibody is specific for SerpinB2 (but not the shorter form).
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide based on the aminoterminal end of mature human SerpinB2

Properties

Applications

Our Abpromise guarantee covers the use of ab47742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notes
    IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 46 kDa. Dilution optimised using Chromogenic detection.

    Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.
    • Sequence similarities
      Belongs to the serpin family. Ov-serpin subfamily.
    • Post-translational
      modifications
      The signal sequence is not cleaved.
    • Cellular localization
      Cytoplasm. Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • HsT1201 antibody
      • Monocyte Arg serpin antibody
      • Monocyte Arg-serpin antibody
      • Monocyte Arginine-serpin antibody
      • Monocyte-derived plasminogen activator inhibitor antibody
      • PAI antibody
      • PAI-2 antibody
      • PAI2 antibody
      • PAI2_HUMAN antibody
      • Placental plasminogen activator inhibitor antibody
      • PLANH2 antibody
      • Plasminogen activator inhibitor 2 antibody
      • plasminogen activator inhibitor, type 2 antibody
      • plasminogen activator inhibitor, type II (arginine-serpin) antibody
      • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2 antibody
      • Serpin B2 antibody
      • serpin peptidase inhibitor, clade B (ovalbumin), member 2 antibody
      • Serpinb2 antibody
      • Urokinase inhibitor antibody
      see all

    Images

    • Ab47742 staining human normal temporal lobe. Staining is localized to the cytoplasm.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Anti-SerpinB2 antibody (ab47742) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 46 kDa
      Observed band size : 49 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute

    References

    This product has been referenced in:
    • Mertens TCJ  et al. Cigarette smoke differentially affects IL-13-induced gene expression in human airway epithelial cells. Physiol Rep 5:N/A (2017). WB . Read more (PubMed: 28701525) »
    • Peng H  et al. microRNA-31/factor-inhibiting hypoxia-inducible factor 1 nexus regulates keratinocyte differentiation. Proc Natl Acad Sci U S A 109:14030-4 (2012). WB, IHC-P ; Human . Read more (PubMed: 22891326) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    100000 cells
    Gel Running Conditions
    Reduced Denaturing (4-20% tris-Glycin gel)
    Sample
    Human Cell lysate - whole cell (Bxpc3)
    Specification
    Bxpc3
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 12 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up