Overview

  • Product name
  • Description
    Mouse monoclonal to SerpinB3
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 276-391 of Human SerpinB3

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab154971 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab55733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 23185467
IHC-P Use at an assay dependent concentration. PubMed: 23185467

Target

  • Function
    May act as a protease inhibitor to modulate the host immune response against tumor cells.
  • Tissue specificity
    Squamous cells. Expressed in some hepatocellular carcinoma (at protein level).
  • Sequence similarities
    Belongs to the serpin family. Ov-serpin subfamily.
  • Developmental stage
    Its expression is closely related to cellular differentiation in both normal and malignant squamous cells.
  • Cellular localization
    Cytoplasm. Seems to also be secreted in plasma by cancerous cells but at a low level.
  • Information by UniProt
  • Database links
  • Alternative names
    • HsT1196 antibody
    • Protein T4 A antibody
    • Protein T4-A antibody
    • SCC antibody
    • SCCA antibody
    • SCCA PD antibody
    • SCCA-1 antibody
    • SCCA-PD antibody
    • SCCA1 antibody
    • SCCAPD antibody
    • Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 3 antibody
    • Serpin B3 antibody
    • Serpin peptidase inhibitor clade B (ovalbumin) member 3 antibody
    • serpin peptidase inhibitor, clade B (ovalbumin), member 3 antibody
    • SERPINB3 antibody
    • SPB3_HUMAN antibody
    • Squamous cell carcinoma antigen 1 antibody
    • T4 A antibody
    • T4-A antibody
    • T4A antibody
    see all

Images

  • Overlay histogram showing A431 cells stained with ab55733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55733, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot against tagged recombinant protein immunogen using ab55733 SerpinB3 antibody at 1ug/ml. Predicted band size of immunogen is 39 kDa

References

This product has been referenced in:
  • Lim W  et al. SERPINB3 in the Chicken Model of Ovarian Cancer: A Prognostic Factor for Platinum Resistance and Survival in Patients with Epithelial Ovarian Cancer. PLoS One 7:e49869 (2012). ICC/IF, IHC-P ; Human, Chicken . Read more (PubMed: 23185467) »
  • Cooksley-Decasper S  et al. Antibody phage display assisted identification of junction plakoglobin as a potential biomarker for atherosclerosis. PLoS One 7:e47985 (2012). WB, IHC . Read more (PubMed: 23110151) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

I can confirm that although we have not tested these antibodies for cross-reactivity with Serpin B4 but there is high percentage of immunogen sequence homology. It means the chances of cross reactivity are high. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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