Overview

  • Product name
  • Description
    Goat polyclonal to SerpinB6
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide:

    NWDEQFDKENTEER

    (Human) from the internal region of the protein sequence according to NP_004559.4.

  • Positive control
    • Human ovary lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/128000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May be involved in the regulation of serine proteinases present in the brain or extravasated from the blood (By similarity). Inhibitor of cathepsin G, kallikrein-8 and thrombin. May play an important role in the inner ear in the protection against leakage of lysosomal content during stress and loss of this protection results in cell death and sensorineural hearing loss.
    • Tissue specificity
      Expressed in keratinocytes (at protein level). Highest levels in skeletal muscle. Also found in placenta, cardiac muscle, lung, liver, kidney and pancreas. Expressed in the inner ear hair cells. Expressed abundantly by normal mast cells in different tissues and by mast cells in mastocytoma lesions.
    • Involvement in disease
      Defects in SERPINB6 are the cause of deafness autosomal recessive type 91 (DFNB91) [MIM:613453]. It is a form of non-syndromic deafness characterized by progressive and age-dependent sensorineural hearing loss. Vestibular function is normal.
    • Sequence similarities
      Belongs to the serpin family. Ov-serpin subfamily.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAP antibody
      • Cytoplasmic antiproteinase antibody
      • Peptidase inhibitor 6 antibody
      • PI-6 antibody
      • PI6 antibody
      • Placental thrombin inhibitor antibody
      • Protease inhibitor 6 (placental thrombin inhibitor) antibody
      • PTI antibody
      • Serpin B6 antibody
      • SERPINB6 antibody
      • SPB6_HUMAN antibody
      see all

    Images

    • Anti-SerpinB6 antibody (ab77372) at 0.1 µg/ml + human ovary lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 42 kDa
      Observed band size : 42 kDa

    References

    ab77372 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab77372.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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