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Rabbit polyclonal to Sertad1
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster
A region within a synthetic peptide: MLSKGLKRKR EEEEEKEPLA VDSWWLDPGH TAVAQAPPAV ASSSLFDLSV, corresponding to N terminal amino acids 1-50 of Human Sertad1
WB: Jurkat cell lysate.
IHC-P: human muscle tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Acts at E2F-responsive promoters to integrate signals provided by PHD- and/or bromodomain-containing transcription factors. Stimulates E2F-1/DP-1 transcriptional activity. Renders the activity of cyclin D1/CDK4 resistant to the inhibitory effects of p16(INK4a).
Contains 1 SERTA domain.
Information by UniProt
CDK4 binding protein p34SEI antibody
CDK4 binding protein p34SEI1 antibody
CDK4-binding protein p34SEI1 antibody
Western blot - Sertad1 antibody (ab65446)
Anti-Sertad1 antibody (ab65446) at 5 µg/ml + Jurkat cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sertad1 antibody (ab65446)
ab65446, at 4µg/ml, staining Sertad1 in paraffin embedded human skeletal muscle cells (indicated by arrows) by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"