Overview

  • Product name
  • Description
    Rabbit polyclonal to Sertad1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster
  • Immunogen

    A region within a synthetic peptide: MLSKGLKRKR EEEEEKEPLA VDSWWLDPGH TAVAQAPPAV ASSSLFDLSV, corresponding to N terminal amino acids 1-50 of Human Sertad1

  • Positive control
    • WB: Jurkat cell lysate. IHC-P: human muscle tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Acts at E2F-responsive promoters to integrate signals provided by PHD- and/or bromodomain-containing transcription factors. Stimulates E2F-1/DP-1 transcriptional activity. Renders the activity of cyclin D1/CDK4 resistant to the inhibitory effects of p16(INK4a).
  • Sequence similarities
    Contains 1 SERTA domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDK4 binding protein p34SEI antibody
    • CDK4 binding protein p34SEI1 antibody
    • CDK4-binding protein p34SEI1 antibody
    • SEI-1 antibody
    • SEI1 antibody
    • SERTA domain containing 1 antibody
    • SERTA domain-containing protein 1 antibody
    • SERTAD1 antibody
    • SRTD1_HUMAN antibody
    • Transcriptional regulator interacting with the PHD-bromodomain 1 antibody
    • TRIP Br1 antibody
    • TRIP-Br1 antibody
    see all

Images

  • Anti-Sertad1 antibody (ab65446) at 5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • ab65446, at 4µg/ml, staining Sertad1 in paraffin embedded human skeletal muscle cells (indicated by arrows) by Immunohistochemistry.

References

ab65446 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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