serum Amyloid A antibody [mc1] (ab655)

Product Name 

serum Amyloid A antibody [mc1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [mc1] to serum Amyloid A

Immunogen 

Human Amyloid protein A coupled to horseradish peroxidase and coupled to high molecular weight kininogen.

Reacts with 
(species key)

Hu

Specificity 

Amyloid A Component reacts with native and fixed amyloid fibrils.
It reacts with amyloid deposits in all tissues including kidney and rectum.
Cross-reactivity with serum precursor of protein AA has been observed.

Tested applications 
(see key)

IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC:
For an ABC system, dilute 1:50-100 and incubate for 30-60 minutes at RT.
For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before performing IHC.

Positive control 
(see definition)

Amyloid deposits in kidney.

Cellular localization 

Expressed by the liver; secreted in plasma.

Research areas 

Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins >> Lipoproteins
Cardiovascular >> Blood >> Serum Proteins
Cardiovascular >> Blood >> Acute Phase Reactants

Relevance 

The serum amyloid A (SAA) family comprises a number of differentially expressed lipoproteins, acute phase SAA1 and SAA2, the former being a major component in plasma, and constitutive SAA's (C-SAAs). Although the liver is the primary site of synthesis of both SAA types, extrhepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed it's importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations. SAA rises earlier and more sharply than CRP.

SAA enhances the binding of HDL's to macrophages and thus helps the delivery of lipid to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease process. In addition, recent experiments suggest that SAA may play a "houekeeping" role in normal human tissues. Elevated levels of SAA over time predispose secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissues and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid artheritis.

Amyloidosis is a heterogeneous group of disorders characterized by extracellular deposition of abnormal protein fibrils, which are derived from different proteins.
Almost all patients with amyloidosis have some level of renal disorders.
The immunoperoxidase technique has the advantage of being able to detect amyloid in formalin-fixed paraffin embedded tissues and is a very specific method for identifying amyloid fibril proteins.
Small and minute amounts of amyloid can be detected with both Amyloid P (ab688) and Amyloid A antibodies, and thus could allow earlier treatment before organ damage has occurred.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

mc1

Isotype 

IgG2a

Storage buffer 

Preservative: Proprietary preservative that is not sodium azide or thimerosal.
Constituents: PBS, Tissue Culture Supernatant. pH 7.3, protein carrier.

Form 

Liquid

Storage instructions 

Store at +4°C.

Notes 

The application of both Congo Red and Amyloid A Component in tissues with amyloid deposits has been shown to be superior to Congo Red alone.

References for serum Amyloid A antibody [mc1] (ab655)

  This product has been used in: (two most recent references)

Linke RP et al. High-sensitivity diagnosis of AA amyloidosis using Congo red and immunohistochemistry detects missed amyloid deposits. J Histochem Cytochem 43:863-9 (1995). PubMed: 7642960

Linke RP Monoclonal antibodies against amyloid fibril protein AA. Production, specificity, and use for immunohistochemical localization and classification of AA-type amyloidosis. J Histochem Cytochem 32:322-8 (1984). PubMed: 6363521
If you publish research using ab655 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding serum Amyloid A antibody [mc1]

Customer FAQs regarding serum Amyloid A antibody [mc1]

Protocols for serum Amyloid A antibody [mc1]

Price and availability of products related to serum Amyloid A antibody [mc1]

serum Amyloid A antibody [mc1] - related products: