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Products:Cardiovascular >> Blood >> Acute Phase Reactants
Overview
Product name
Anti-serum Amyloid A antibody [mc1]
See all Serum Amyloid A products (15) ...
Description
Mouse monoclonal [mc1] to serum Amyloid A
Specificity
Amyloid A Component reacts with native and fixed amyloid fibrils. It reacts with amyloid deposits in all tissues including kidney and rectum. Cross-reactivity with serum precursor of protein AA has been observed.
Tested applications
IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Human Amyloid protein A coupled to horseradish peroxidase and coupled to high molecular weight kininogen.
Positive control
Amyloid deposits in kidney.
General notes
The application of both Congo Red and Amyloid A Component in tissues with amyloid deposits has been shown to be superior to Congo Red alone.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: Proprietary preservative that is not sodium azide or thimerosal.
Constituents: PBS, Tissue Culture Supernatant. pH 7.3, protein carrier.
Concentration
Concentration information loading...
Primary antibody notes
Amyloidosis is a heterogeneous group of disorders characterized by extracellular deposition of abnormal protein fibrils, which are derived from different proteins. Almost all patients with amyloidosis have some level of renal disorders. The immunoperoxidase technique has the advantage of being able to detect amyloid in formalin-fixed paraffin embedded tissues and is a very specific method for identifying amyloid fibril proteins. Small and minute amounts of amyloid can be detected with both Amyloid P (ab688) and Amyloid A antibodies, and thus could allow earlier treatment before organ damage has occurred.
Clonality
Monoclonal
Clone number
mc1
Myeloma
unknown
Isotype
IgG2a
Light chain type
unknown
Research areas
Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins >> Lipoproteins
Cardiovascular >> Blood >> Serum Proteins
Cardiovascular >> Blood >> Acute Phase Reactants
Applications
Show applications key
Our Abpromise guarantee covers the use of ab655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
Application notes
IHC:
For an ABC system, dilute 1:50-100 and incubate for 30-60 minutes at RT.
For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before performing IHC.
Target
Function
Major acute phase reactant. Apolipoprotein of the HDL complex.
Tissue specificity
Expressed by the liver; secreted in plasma.
Involvement in disease
Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
Note=Elevated serum SSA protein levels may be associated with lung cancer.
Sequence similarities
Belongs to the SAA family.
Post-translational
modifications
This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
Cellular localization
Secreted.
Target information above from: UniProt accessionP02735
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amyloid fibril protein AA antibody
- Amyloid protein A antibody
- MGC111216 antibody
see all
Database links
- Entrez Gene: 6288 Human
- Entrez Gene: 6289 Human
- Omim: 104750 Human
- SwissProt: P02735 Human
- Unigene: 1955 Human
- Unigene: 632144 Human
References for Anti-serum Amyloid A antibody [mc1] (ab655)
This product has been referenced in:
- Paret Cet al. Inflammatory Protein Serum Amyloid A1 Marks a Subset of Conventional Renal Cell Carcinomas with Fatal Outcome. Eur Urol : (2009).Read more (PubMed: 19747761) »
- Linke RPet al. High-sensitivity diagnosis of AA amyloidosis using Congo red and immunohistochemistry detects missed amyloid deposits. J Histochem Cytochem 43:863-9 (1995).Read more (PubMed: 7642960) »
See all 3 publications for this product
Publishing research using ab655? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"