Anti-Serum Amyloid A antibody [115] (ab687)

Overview

  • Product name
    Anti-Serum Amyloid A antibody [115]
    See all Serum Amyloid A primary antibodies
  • Description
    Mouse monoclonal [115] to Serum Amyloid A
  • Specificity
    This antibody reacts with natural and recombinant human Serum Amyloid A (SAA) and does not cross-react with other human cytokines or growth factors tested such as IL-1beta, IL-8, MCAF, TGF-beta and EGF.
  • Tested applications
    Suitable for: WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly purified recombinant human serum amyloid A (MW: 12 kDa).

Properties

Applications

Our Abpromise guarantee covers the use of ab687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Use at an assay dependent dilution. This antibody, at a concentration of 0.1-0.5 µg/ml, will allow visualisation of 100 ng/lane of recombinant human SAA.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA Use at an assay dependent concentration. sELISA compatible pair

Target

  • Function
    Major acute phase reactant. Apolipoprotein of the HDL complex.
  • Tissue specificity
    Expressed by the liver; secreted in plasma.
  • Involvement in disease
    Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
    Note=Elevated serum SSA protein levels may be associated with lung cancer.
  • Sequence similarities
    Belongs to the SAA family.
  • Post-translational
    modifications
    This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amyloid fibril protein AA antibody
    • Amyloid fibrils antibody
    • Amyloid protein A antibody
    • Fibrils antibody
    • MGC111216 antibody
    • OC antibody
    • PIG 4 antibody
    • PIG4 antibody
    • SAA 1 antibody
    • SAA 2 antibody
    • SAA antibody
    • SAA_HUMAN antibody
    • SAA1 antibody
    • SAA2 antibody
    • Serum amyloid A protein precursor antibody
    • Serum amyloid A1 isoform 1 antibody
    • Serum amyloid A1 isoform 2 antibody
    • Serum amyloid protein A(4-101) antibody
    • TP53I4 antibody
    • Tumor protein p53 inducible protein 4 antibody
    see all

Images

  • All lanes : Anti-Serum Amyloid A antibody [115] (ab687) at 1/4000 dilution

    Lane 1 : Acute phase plasma samples
    Lane 2 : Acute phase plasma samples
    Lane 3 : Acute phase plasma samples
    Lane 4 : Acute phase plasma samples
    Lane 5 : Recombinant C-SAA
    Lane 6 : Recombinant A-SAA

    Lysates/proteins at 14 µg per lane.

    Secondary
    Goat anti-mouse IgG-HRP at 1/10000 dilution
    Developed using the ECL technique

    Exposure time : 5 seconds

    Image courtesy of an anonymous Abreview.

  • Ab687 staining human normal liver. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

This product has been referenced in:
  • Heo SH  et al. Comparative proteomic analysis in children with idiopathic short stature (ISS) before and after short-term recombinant human growth hormone (rhGH) therapy. Proteomics 13:1211-9 (2013). Human . Read more (PubMed: 23436750) »
  • Jahangiri A  et al. Serum amyloid A is found on ApoB-containing lipoproteins in obese humans with diabetes. Obesity (Silver Spring) 21:993-6 (2013). Read more (PubMed: 23784902) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako
Permeabilization
No
Specification
Liver
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 13 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Blocking
Sample
Human Cultured Cells (monocytic cells)
Specification
monocytic cells
Username

Abcam user community

Verified customer

Submitted Sep 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Heart, Left Ventricle)
Loading amount
80 µg
Specification
Heart, Left Ventricle
Gel Running Conditions
Reduced Denaturing (10-20% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dawn Bowles

Verified customer

Submitted Dec 28 2012

Thank you for contacting us.

I have now received further information from developer lab regarding the products ab687 and ab18711. Regrettably we will not be able to recommend these antibodies, to be used with horse samples. I apologies for any...

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Thank you for your email.

Finally, I have checked the sequence homology between human and horse SAA proteins, the homology is quite low with matching stretches.

http://www.uniprot.org/blast/uniprot/2012101621EV411P6G?alignment=27 Read More

Thank you for contacting us.

We can recommend paring between ab687 and ab18711 instead of ab18713

http://www.abcam.com/serum-Amyloid-A-antibody-607-ab18711.html

http://www.abcam.com/Serum-Amyloid-A-antibody-115-ab687.html
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Thank you for contacting us. The antibody ab687 is reactive towards the Serum Amyloid A protein and not specific variants.. We do not have any antibodies specific for SAA1,SAA2 or SAA4.

I hope this information is helpful to you. Please do not ...

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Thank you for contacting us.

I have looked into what applications anti-Serum Amyloid A antibodies ab687 and ab81483 have been used for in order to ascertain if they are likely to detect the native epitope of Serum Amyloid A in order to perfor...

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Thank you for your phone call. 1) Regarding the image for ab1793, this was submitted by a customer as Abreview. For the details of the Abreview please follow this link: http://www.abcam.com/index.html?datasheet=1793&tab=abreviews&intabreviewid=17818...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (whole human acute phase plasma)
Loading amount
14 µg
Specification
whole human acute phase plasma
Treatment
heated to 95C in 10-fold excess of reducing SDS sample buffer for 10min
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 21 2011

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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